2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. L82

L82 is a selective and uncompetitive DNA ligase 1 (DNA Lig1) inhibitor (hLig1 IC50=12 μM). L82 shows anti-proliferative activity to breast cancer cells.

For research use only. We do not sell to patients.

L82 Chemical Structure

L82 Chemical Structure

CAS No. : 329227-30-7

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Based on 1 publication(s) in Google Scholar

L82 Related Antibodies

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Description

L82 is a selective and uncompetitive DNA ligase 1 (DNA Lig1) inhibitor (hLig1 IC50=12 μM). L82 shows anti-proliferative activity to breast cancer cells[1][2].

In Vitro

L82 (0-50 μM; 6 d) shows anti-proliferative activity to breast cancer cells[2].
L82 (50 μM; 0-48 h) shows cytostatic activity due to activation of the G1/S checkpoint in MCF7 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L82 Related Antibodies

Cell Proliferation Assay[2]

Cell Line: MCF10A, MCF7, HCT116, and HeLa cells
Concentration: 0-50 μM
Incubation Time: 6 days
Result: Reduced the proliferation of a normal breast epithelial cell line MCF10A and the breast cancer cell lines MCF7, HeLa and HCT116, in a concentration-dependent manner.

Cell Cycle Analysis[2]

Cell Line: MCF7 cells
Concentration: 50 μM
Incubation Time: 0-48 hours
Result: Showed a transient accumulation of cells at G2/M after 12 h, then showed an accumulation at G0/G1 that peaked after 24 h.
Decreased in the S phase cell in accompany with the increase in the G0/G1 phase.
Molecular Weight

309.67

Formula

C11H8ClN5O4
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O=C1NN=CC(N/N=C/C2=CC=C(C([N+]([O-])=O)=C2)O)=C1Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (107.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2292 mL 16.1462 mL 32.2924 mL
5 mM 0.6458 mL 3.2292 mL 6.4585 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2292 mL 16.1462 mL 32.2924 mL 80.7311 mL
5 mM 0.6458 mL 3.2292 mL 6.4585 mL 16.1462 mL
10 mM 0.3229 mL 1.6146 mL 3.2292 mL 8.0731 mL
15 mM 0.2153 mL 1.0764 mL 2.1528 mL 5.3821 mL
20 mM 0.1615 mL 0.8073 mL 1.6146 mL 4.0366 mL
25 mM 0.1292 mL 0.6458 mL 1.2917 mL 3.2292 mL
30 mM 0.1076 mL 0.5382 mL 1.0764 mL 2.6910 mL
40 mM 0.0807 mL 0.4037 mL 0.8073 mL 2.0183 mL
50 mM 0.0646 mL 0.3229 mL 0.6458 mL 1.6146 mL
60 mM 0.0538 mL 0.2691 mL 0.5382 mL 1.3455 mL
80 mM 0.0404 mL 0.2018 mL 0.4037 mL 1.0091 mL
100 mM 0.0323 mL 0.1615 mL 0.3229 mL 0.8073 mL
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L82 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L82329227-30-7L 82L-82DNA/RNA Synthesisanti-proliferative activityMCF10AMCF7HCT116HeLaInhibitorinhibitorinhibit

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