Linoleoyl ethanolamide (Synonyms: Linoleic acid monoethanolamide)

Name: Linoleoyl ethanolamide
Brand: MedChemExpress
SKU: HY-113421
Rating: 4.5 (1 reviews)

Cat. No.: HY-113421 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Linoleoyl ethanolamide (Linoleic acid monoethanolamide) is classified as a fatty acid ethanolamide. Linoleoyl ethanolamide only weakly binds G-protein-coupled cannabinoid receptors of type-1（CB1）and CB2 receptors, and inhibits the binding of ^[3H]CP-55,940 with K_is of 10 and 25 μM, respectively. Linoleoyl ethanolamide is 4-fold less potent than anandamide at causing catalepsy in mice and it does not prolong sleep time.

For research use only. We do not sell to patients.

Linoleoyl ethanolamide Chemical Structure

CAS No. : 68171-52-8

Size	Price	Stock	Quantity
5 mg	USD 98	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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CB1 CB2

Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
RBL-2H3	IC₅₀	48 μM Compound: 11	Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2 Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2	[PMID: 31618024]

In Vitro

Linoleoyl ethanolamide competitively inhibits the hydrolysis of anandamide and may be involved in the regulation of food intake by selective prolongation of feeding latency and post-meal interval^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Linoleoyl ethanolamide (0.1 mg-0.2 mg; challenged by painting the inner and outer surfaces of both sides of their ears) suppresses DNFB-induced ear swelling in BALB/c mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

323.51

Formula

C₂₀H₃₇NO₂

CAS No.

68171-52-8

Appearance

Oil

Color

Light yellow to light brown

SMILES

CCCCC/C=C\C/C=C\CCCCCCCC(NCCO)=O

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (309.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0911 mL	15.4555 mL	30.9109 mL
5 mM	0.6182 mL	3.0911 mL	6.1822 mL
10 mM	0.3091 mL	1.5455 mL	3.0911 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (266 KB) SDS (545 KB)

COA (238 KB) HNMR (248 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Wang X, et al. X. Synthesis of linoleoyl ethanolamide. J Oleo Sci. 2013;62(6):427-433. [Content Brief]

[2]. Lin S, et al. Novel analogues of arachidonylethanolamide (anandamide): affinities for the CB1 and CB2 cannabinoid receptors and metabolic stability. J Med Chem. 1998;41(27):5353-5361. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0911 mL	15.4555 mL	30.9109 mL	77.2774 mL
	5 mM	0.6182 mL	3.0911 mL	6.1822 mL	15.4555 mL
	10 mM	0.3091 mL	1.5455 mL	3.0911 mL	7.7277 mL
	15 mM	0.2061 mL	1.0304 mL	2.0607 mL	5.1518 mL
	20 mM	0.1546 mL	0.7728 mL	1.5455 mL	3.8639 mL
	25 mM	0.1236 mL	0.6182 mL	1.2364 mL	3.0911 mL
	30 mM	0.1030 mL	0.5152 mL	1.0304 mL	2.5759 mL
	40 mM	0.0773 mL	0.3864 mL	0.7728 mL	1.9319 mL
	50 mM	0.0618 mL	0.3091 mL	0.6182 mL	1.5455 mL
	60 mM	0.0515 mL	0.2576 mL	0.5152 mL	1.2880 mL
	80 mM	0.0386 mL	0.1932 mL	0.3864 mL	0.9660 mL
	100 mM	0.0309 mL	0.1546 mL	0.3091 mL	0.7728 mL

Linoleoyl ethanolamide Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Linoleoyl ethanolamide68171-52-8Linoleic acid monoethanolamideCannabinoid Receptoranandamidefoodintakeprolongationfeedingpost-mealGproteincoupledInhibitorinhibitorinhibit

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Linoleoyl ethanolamide (Synonyms: Linoleic acid monoethanolamide)

Customer Review

Linoleoyl ethanolamide Related Antibodies

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•Related Small Molecules:

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Biological Activity

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References

Customer Review

Linoleoyl ethanolamide Related Antibodies

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Complete Stock Solution Preparation Table

Linoleoyl ethanolamide Related Classifications

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