2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. Lumigen APS-5

Lumigen APS-5 is a substrate of alkaline phosphatase (ALP). Lumigen APS-5 can be used to assess the activity of alkaline phosphatase (ALP).

For research use only. We do not sell to patients.

Lumigen APS-5 Chemical Structure

Lumigen APS-5 Chemical Structure

CAS No. : 193884-53-6

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Based on 1 publication(s) in Google Scholar

Lumigen APS-5 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

Lumigen APS-5 is a substrate of alkaline phosphatase (ALP). Lumigen APS-5 can be used to assess the activity of alkaline phosphatase (ALP)[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Measurement of Alkaline Phosphatase Activity[1]:
1. Mix testing samples (10 mg) with Lumigen APS-5 (100 μL) and vortexed for 10 s at the highest setting. For the control, 10 μL of distilled water.
2. The mixed samples are heat treated at 75℃ for 1 min in a water bath to inactivate nonbacterial ALP. After heat treatment, samples are placed in 96 well plates and measured immediately. The activity of ALP is measured by using a luminometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lumigen APS-5 Related Antibodies
Molecular Weight

489.82

Formula

C21H15ClNNa2O4PS
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

ClC(C=C1)=CC=C1S/C(OP(O[Na])(O[Na])=O)=C2C3=C(N(C)C4=C\2C=CC=C4)C=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 5.83 mg/mL (11.90 mM; ultrasonic and warming and heat to 60°C)

DMSO : 3.33 mg/mL (6.80 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0416 mL 10.2078 mL 20.4157 mL
5 mM 0.4083 mL 2.0416 mL 4.0831 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

SDS (419 KB)

COA (257 KB) HNMR (278 KB) CNMR (284 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.0416 mL 10.2078 mL 20.4157 mL 51.0392 mL
5 mM 0.4083 mL 2.0416 mL 4.0831 mL 10.2078 mL
H2O 10 mM 0.2042 mL 1.0208 mL 2.0416 mL 5.1039 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Lumigen APS-5 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lumigen APS-5193884-53-6Lumigen APS5Lumigen APS 5PhosphatasesubstrateactivityALPInhibitorinhibitorinhibit

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