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  4. Magnesium chloride hexahydrate, for molecular biology

Magnesium chloride hexahydrate, for molecular biology  (Synonyms: MgCl2 hexahydrate, for molecular biology)

Cat. No.: HY-W019981B Purity: 99.40%
Data Sheet
SDS
COA
Handling Instructions Technical Support

Solubility

In Vivo Dissolution Calculator

Help & FAQs

Magnesium chloride hexahydrate (MgCl2 hexahydrate), for molecular biology is the hexahydrate form of magnesium dichloride. Magnesium chloride hexahydrate can be used as a source of magnesium ion and a co-foactor for many enzymes. Magnesium chloride hexahydrate increases the activity of Ca2+ ATPase.

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Magnesium chloride hexahydrate, for molecular biology Chemical Structure

Magnesium chloride hexahydrate, for molecular biology Chemical Structure

CAS No. : 7791-18-6

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Description

Magnesium chloride hexahydrate (MgCl2 hexahydrate), for molecular biology is the hexahydrate form of magnesium dichloride. Magnesium chloride hexahydrate can be used as a source of magnesium ion and a co-foactor for many enzymes. Magnesium chloride hexahydrate increases the activity of Ca2+ ATPase[1].

In Vitro

Magnesium chloride (MgCl2) neutralized effects of chlorpyrifos by promoting normal ATPase activities and inhibiting release of acetylcholine from cell[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

203.30

Formula

Cl2H12MgO6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

Cl[Mg]Cl.O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature, keep dry and cool

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (491.88 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.9188 mL 24.5942 mL 49.1884 mL
5 mM 0.9838 mL 4.9188 mL 9.8377 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Number of animals

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Purity & Documentation

Purity: 99.40%

Data Sheet (270 KB) SDS (393 KB)

COA (247 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 4.9188 mL 24.5942 mL 49.1884 mL 122.9710 mL
5 mM 0.9838 mL 4.9188 mL 9.8377 mL 24.5942 mL
10 mM 0.4919 mL 2.4594 mL 4.9188 mL 12.2971 mL
15 mM 0.3279 mL 1.6396 mL 3.2792 mL 8.1981 mL
20 mM 0.2459 mL 1.2297 mL 2.4594 mL 6.1485 mL
25 mM 0.1968 mL 0.9838 mL 1.9675 mL 4.9188 mL
30 mM 0.1640 mL 0.8198 mL 1.6396 mL 4.0990 mL
40 mM 0.1230 mL 0.6149 mL 1.2297 mL 3.0743 mL
50 mM 0.0984 mL 0.4919 mL 0.9838 mL 2.4594 mL
60 mM 0.0820 mL 0.4099 mL 0.8198 mL 2.0495 mL
80 mM 0.0615 mL 0.3074 mL 0.6149 mL 1.5371 mL
100 mM 0.0492 mL 0.2459 mL 0.4919 mL 1.2297 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Magnesium chloride hexahydrate, for molecular biology Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

Keywords:

Magnesium chloride hexahydrate, for molecular biology7791-18-6MgCl2 hexahydrate, for molecular biologyCalcium ChannelBiochemical Assay ReagentsCa2+ channelsCa channelsbiochemical reagentmolecular biologyInhibitorinhibitorinhibit

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