Mal-C2-cyclohexylcarboxyl-hydrazide hydrochloride

Name: Mal-C2-cyclohexylcarboxyl-hydrazide hydrochloride
Brand: MedChemExpress
SKU: HY-133410A
Rating: 4.5 (1 reviews)

Cat. No.: HY-133410A Purity: 98.23%: FAQs
Data Sheet SDS COA Handling Instructions

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Mal-C2-cyclohexylcarboxyl-hydrazide hydrochloride is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs.

For research use only. We do not sell to patients.

Mal-C2-cyclohexylcarboxyl-hydrazide hydrochloride Chemical Structure

CAS No. : 174422-72-1

Size	Price	Stock	Quantity
25 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Mal-C2-cyclohexylcarboxyl-hydrazide hydrochloride:

Mal-C2-cyclohexylcarboxyl-hydrazide TFA Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Mal-C2-cyclohexylcarboxyl-hydrazide hydrochloride is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs^[1].

IC₅₀ & Target

Alkyl-Chain

In Vitro

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

287.74

Formula

C₁₂H₁₈ClN₃O₃

CAS No.

174422-72-1

Appearance

Solid

Color

White to off-white

SMILES

Cl[H].O=C1C=CC(N1CC(CC2)CCC2C(NN)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 33.33 mg/mL (115.83 mM; Need ultrasonic)

DMSO : 16.67 mg/mL (57.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4754 mL	17.3768 mL	34.7536 mL
5 mM	0.6951 mL	3.4754 mL	6.9507 mL
10 mM	0.3475 mL	1.7377 mL	3.4754 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (7.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.23%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (249 KB) HNMR (271 KB) LCMS (102 KB)

Handling Instructions (2659 KB)

References

[1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.4754 mL	17.3768 mL	34.7536 mL	86.8840 mL
	5 mM	0.6951 mL	3.4754 mL	6.9507 mL	17.3768 mL
	10 mM	0.3475 mL	1.7377 mL	3.4754 mL	8.6884 mL
	15 mM	0.2317 mL	1.1585 mL	2.3169 mL	5.7923 mL
	20 mM	0.1738 mL	0.8688 mL	1.7377 mL	4.3442 mL
	25 mM	0.1390 mL	0.6951 mL	1.3901 mL	3.4754 mL
	30 mM	0.1158 mL	0.5792 mL	1.1585 mL	2.8961 mL
	40 mM	0.0869 mL	0.4344 mL	0.8688 mL	2.1721 mL
	50 mM	0.0695 mL	0.3475 mL	0.6951 mL	1.7377 mL
H₂O	60 mM	0.0579 mL	0.2896 mL	0.5792 mL	1.4481 mL
	80 mM	0.0434 mL	0.2172 mL	0.4344 mL	1.0860 mL
	100 mM	0.0348 mL	0.1738 mL	0.3475 mL	0.8688 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.