2. Cytoskeleton Epigenetics TGF-beta/Smad
  3. MARCKS PKC
  4. MANS peptide TFA

MANS peptide TFA is the TFA salt form of MANS peptide (HY-P10218). MANS peptide TFA is an inhibitor for myristoylated alanine-rich C kinase substrate (MARCKS), which competes with MARCKS in cells for membrane binding, and thus inhibits the stimulation of mucin secretion and tumor metastasis.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

MANS peptide TFA Chemical Structure

MANS peptide TFA Chemical Structure

Size Price Stock Quantity
5 mg USD 420 In-stock
10 mg USD 700 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of MANS peptide TFA:

MANS peptide TFA Related Antibodies

Top Publications Citing Use of Products

View All PKC Isoform Specific Products:

View All Isoforms
PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

MANS peptide TFA is the TFA salt form of MANS peptide (HY-P10218). MANS peptide TFA is an inhibitor for myristoylated alanine-rich C kinase substrate (MARCKS), which competes with MARCKS in cells for membrane binding, and thus inhibits the stimulation of mucin secretion and tumor metastasis[1].

In Vitro

MANS peptide TFA (0-100 μM, 12-24 h) inhibits migration and invasion of lung cancer cells CL1-0/F3, CL1-5, PC9 and A549 without causing toxicity to normal cells[1].
MANS peptide TFA (0-100 μM, 16 h) inihibits MARCKS phosphorylation and PI3K and AKT phosphorylation, leads to downstream changes in Slug and E-cadherin expression levels, prevents the loss of cell-cell adhesion, alters epithelial-mesenchymal transition (EMT) characteristics of cancer cells, and thus decreases tumor metastasis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MANS peptide TFA Related Antibodies

Cell Migration Assay [1]

Cell Line: CL1-0/F3, CL1-5 and PC9
Concentration: 0-100 μM
Incubation Time: 12-24 h
Result: Inhibited migration.

Western Blot Analysis[1]

Cell Line: CL1-0/F3, CL1-5, PC9 and NHBE
Concentration: 0-100 μM
Incubation Time: 16 h
Result: Upregulated levels of E-cadherin, downregulated levels of Slug.
Suppressed MARCKS phosphorylation and AKT/Slug pathway.
In Vivo

MANS peptide TFA (50 nmol/injection, ip, every 3 days for 6 injection) inhibits tumor metastasis, without affecting tumorigenesis in PC9 xenograft NOD/SCID mice model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NOD/SCID mice model[1]
Dosage: 50 nmol/injection
Administration: Ip, every 3 days for 6 times
Result: Suppressed micrometastatic lesions.
Molecular Weight

2498.83 (free acid)

Formula

C111H184N30O35.xC2HF3O2
Appearance

Solid

Color

White to off-white

Sequence

{Myristic acid-Gly}-Ala-Gln-Phe-Ser-Lys-Thr-Ala-Ala-Lys-Gly-Glu-Ala-Ala-Ala-Glu-Arg-Pro-Gly-Glu-Ala-Ala-Val-Ala
Sequence Shortening

{Myristic acid-Gly}-AQFSKTAAKGEAAAERPGEAAVA
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 13.3 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 96.06%

SDS (252 KB)

COA (258 KB) RP-HPLC (112 KB) MS (208 KB)

Handling Instructions (2659 KB)
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

MANS peptide TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MANS peptideMARCKSPKCMyristoylated alanine-rich C kinase substrateProtein kinase CCL1-0/F3CL1-5PC9A549NOD/SCID miceInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
MANS peptide TFA
Cat. No.:
HY-P10218A
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.