MBM-55

Name: MBM-55
Brand: MedChemExpress
SKU: HY-101029
Rating: 4.5 (1 reviews)

Cat. No.: HY-101029 Purity: 99.17%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

MBM-55 is a potent NIMA-related kinase 2 (Nek2) inhibitor with an IC₅₀ of 1 nM. MBM-55 shows a 20-fold or greater selectivity in most kinases with the exception of RSK1 (IC₅₀=5.4 nM) and DYRK1a (IC₅₀=6.5 nM). MBM-55 effectively inhibits the proliferation of cancer cells by inducing cell cycle arrest and apoptosis. MBM-55 shows antitumor activities, and no obvious toxicity to mice.

For research use only. We do not sell to patients.

MBM-55 Chemical Structure

CAS No. : 2083622-09-5

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 417	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 417	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 228	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 840	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1100	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1500	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of MBM-55:

MBM-55S Get quote

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NEK1 NEK2 NEK6 NEK7

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

NEK2

1 nM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
Bel-7402	IC₅₀	6.37 μM Compound: MBM-55	Antitumor activity against human Bel-7402 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay Antitumor activity against human Bel-7402 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay	[PMID: 32992252]
Bel-7402	IC₅₀	7.13 μM Compound: 42g; MBM-55	Cytotoxicity against human Bel7402 cells measured after 72 hrs by MTT assay Cytotoxicity against human Bel7402 cells measured after 72 hrs by MTT assay	[PMID: 28039836]
HCT-116	IC₅₀	0.7 μM Compound: MBM-55	Antitumor activity against human HCT-116 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay Antitumor activity against human HCT-116 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay	[PMID: 32992252]
HCT-116	IC₅₀	0.84 μM Compound: 42g; MBM-55	Cytotoxicity against human HCT116 cells measured after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells measured after 72 hrs by MTT assay	[PMID: 28039836]
Hep 3B2	IC₅₀	1.07 μM Compound: MBM-55	Antitumor activity against human Hep3B cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay Antitumor activity against human Hep3B cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay	[PMID: 32992252]
MGC-803	IC₅₀	0.53 μM Compound: 42g; MBM-55	Cytotoxicity against human MGC803 cells measured after 72 hrs by MTT assay Cytotoxicity against human MGC803 cells measured after 72 hrs by MTT assay	[PMID: 28039836]
MGC-803	IC₅₀	0.65 μM Compound: MBM-55	Antitumor activity against human MGC-803 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay Antitumor activity against human MGC-803 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay	[PMID: 32992252]

In Vitro

MBM-55 (compound 42g) inhibits MGC-803, HCT-116, Bel-7402 cells proliferation with IC₅₀s of 0.53, 0.84, 7.13 μM, respectively^[1].
MBM-55 (0.5-1 μM; 24 hours) induces G2/M phase arrest and accumulation of cells with >4N content in HCT-116 cells^[1].
MBM-55 (0.5-1 μM; 24 hours) causes cell apoptosis in a concentration-dependent manner in HCT-116 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:	HCT-116 cells
Concentration:	0.5, 1 μM
Incubation Time:	24 hours
Result:	Induced G2/M phase arrest and accumulation of cells with >4N content.

Apoptosis Analysis^[1]

Cell Line:	HCT-116 cells
Concentration:	0.5, 1 μM
Incubation Time:	24 hours
Result:	Caused cell apoptosis in a concentration-dependent manner.

In Vivo

MBM-55 (20 mg/kg; i.p.; twice a day for 21 days) exhibits good antitumor activity and a well-tolerated dose schedule in nude mice bearing HCT-116 xenografts^[1].
MBM-55 (1.0 mg/kg; i.v.) treatment shows the CL, V_ss, T_1/2, AUC_0-t, and AUC_0-∞ values of 33.3 mL/min/kg, 2.53 L/kg, 1.72 hours, 495 ng/h/mL and 507 ng/h/mL, respectively.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female BALB/c nu/nu mice (5-6 weeks, bearing HCT-116 xenografts)^[1]
Dosage:	20 mg/kg
Administration:	Intraperitoneal injection; twice a day for 21 days
Result:	Significantly suppressed tumor growth.

Animal Model:	Male Sprague Dawley (SD) rats^[1]
Dosage:	1.0 mg/kg
Administration:	IV injection (Pharmacokinetic Analysis)
Result:	The CL, V_ss, T_1/2, AUC_0-t, and AUC_0-∞ values were 33.3 mL/min/kg, 2.53 L/kg, 1.72 hours, 495 ng/h/mL and 507 ng/h/mL, respectively.

Molecular Weight

498.55

Formula

C₂₈H₂₇FN₆O₂

CAS No.

2083622-09-5

Appearance

Solid

Color

White to off-white

SMILES

NC(C(C=C1)=C(OCC2=CC=CC(F)=C2)C=C1C3=CN=C4N3C=CC(C5=CN(CCN(C)C)N=C5)=C4)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (250.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0058 mL	10.0291 mL	20.0582 mL
5 mM	0.4012 mL	2.0058 mL	4.0116 mL
10 mM	0.2006 mL	1.0029 mL	2.0058 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (275 KB) SDS (252 KB)

COA (249 KB) HNMR (288 KB) LCMS (270 KB)

Handling Instructions (2659 KB)

References

[1]. Xi JB, et al. Structure-based design and synthesis of imidazo[1,2-a]pyridine derivatives as novel and potent Nek2 inhibitors with in vitro and in vivo antitumor activities. Eur J Med Chem. 2017 Jan 27;126:1083-1106. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0058 mL	10.0291 mL	20.0582 mL	50.1454 mL
	5 mM	0.4012 mL	2.0058 mL	4.0116 mL	10.0291 mL
	10 mM	0.2006 mL	1.0029 mL	2.0058 mL	5.0145 mL
	15 mM	0.1337 mL	0.6686 mL	1.3372 mL	3.3430 mL
	20 mM	0.1003 mL	0.5015 mL	1.0029 mL	2.5073 mL
	25 mM	0.0802 mL	0.4012 mL	0.8023 mL	2.0058 mL
	30 mM	0.0669 mL	0.3343 mL	0.6686 mL	1.6715 mL
	40 mM	0.0501 mL	0.2507 mL	0.5015 mL	1.2536 mL
	50 mM	0.0401 mL	0.2006 mL	0.4012 mL	1.0029 mL
	60 mM	0.0334 mL	0.1672 mL	0.3343 mL	0.8358 mL
	80 mM	0.0251 mL	0.1254 mL	0.2507 mL	0.6268 mL
	100 mM	0.0201 mL	0.1003 mL	0.2006 mL	0.5015 mL

MBM-55 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MBM-552083622-09-5MBM55MBM 55NEKsApoptosisNIMA–related KinasesNIMA (never in mitosis gene a)-related Expressed KinasesantitumorselectivityNek2proliferationcellcyclearrestapoptosisInhibitorinhibitorinhibit

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MBM-55

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Other Forms of MBM-55:

MBM-55 Related Antibodies

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MBM-55 Related Antibodies

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Complete Stock Solution Preparation Table

MBM-55 Related Classifications

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