MCA

Cat. No.: HY-W027544 Purity: 98.87%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities.

For research use only. We do not sell to patients.

MCA Chemical Structure

CAS No. : 62935-72-2

Size	Stock	Quantity
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

or Bulk Inquiry

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This product is a controlled substance and not for sale in your territory.

Other Forms of MCA:

(7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d₃ Get quote
(7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d₆ Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Clinical Trial

Molecular Weight

234.20

Formula

C₁₂H₁₀O₅

CAS No.

62935-72-2

Appearance

Solid

Color

White to off-white

Emission (Em)

450

Excitation (Ex)

345

SMILES

O=C1OC(C=C(OC)C=C2)=C2C(CC(O)=O)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (106.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.2699 mL	21.3493 mL	42.6985 mL
5 mM	0.8540 mL	4.2699 mL	8.5397 mL
10 mM	0.4270 mL	2.1349 mL	4.2699 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.87%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (250 KB) HNMR (112 KB) RP-HPLC (230 KB)

Handling Instructions (2659 KB)

References

[1]. Perera NC, et, al. NSP4 is stored in azurophil granules and released by activated neutrophils as active endoprotease with restricted specificity. J Immunol. 2013 Sep 1;191(5):2700-7. [Content Brief]

[2]. Choi YH,et, al. Effect of functional groups on the solubilities of coumarin derivatives in supercritical carbon dioxide. Chromatographia. 1998 Jan;47(93-7).

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.2699 mL	21.3493 mL	42.6985 mL	106.7464 mL
	5 mM	0.8540 mL	4.2699 mL	8.5397 mL	21.3493 mL
	10 mM	0.4270 mL	2.1349 mL	4.2699 mL	10.6746 mL
	15 mM	0.2847 mL	1.4233 mL	2.8466 mL	7.1164 mL
	20 mM	0.2135 mL	1.0675 mL	2.1349 mL	5.3373 mL
	25 mM	0.1708 mL	0.8540 mL	1.7079 mL	4.2699 mL
	30 mM	0.1423 mL	0.7116 mL	1.4233 mL	3.5582 mL
	40 mM	0.1067 mL	0.5337 mL	1.0675 mL	2.6687 mL
	50 mM	0.0854 mL	0.4270 mL	0.8540 mL	2.1349 mL
	60 mM	0.0712 mL	0.3558 mL	0.7116 mL	1.7791 mL
	80 mM	0.0534 mL	0.2669 mL	0.5337 mL	1.3343 mL
	100 mM	0.0427 mL	0.2135 mL	0.4270 mL	1.0675 mL

MCA Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MCA62935-72-2Fluorescent Dyecoumarinplatelet-activating factorhigh-performance liquid chromatographyFRET peptideproteaseInhibitorinhibitorinhibit

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MCA

Other Forms of MCA:

MCA Related Antibodies

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