2. Academic Validation
  3. High expression of serine protease inhibitor kazal type 1 predicts poor prognosis and promotes the progression and invasion of oral tongue squamous cell carcinoma

High expression of serine protease inhibitor kazal type 1 predicts poor prognosis and promotes the progression and invasion of oral tongue squamous cell carcinoma

  • Arch Oral Biol. 2024 May 17:164:106003. doi: 10.1016/j.archoralbio.2024.106003.
Shuang Wang 1,  Yaping Sun 2,  Dan Shao 2,  Yunjie Pan 2,  Xiaoyan Gao 3,  Peng Zhao 2,  Qiaoling Liu 4,  Gaishuang Shang 5,  Wei Shang 6,  Zhiguang Fu 7,  Yong Sun 8
Affiliations

Affiliations

  • 1 Department of Pharmaceutics, School of Pharmacy, Qingdao University, Qingdao 266021, China; Department of Stomatology, Huangdao District Central Hospital, Qingdao 266555, China.
  • 2 Department of Stomatology, Huangdao District Central Hospital, Qingdao 266555, China.
  • 3 Traditional Chinese Medical Hospital of Huangdao District, Qingdao 266499,China.
  • 4 Department of Oncology, Huangdao District Central Hospital, Qingdao 266555, China.
  • 5 Department of Scientific Research, Qingdao East Sea Pharmaceutical Co., Ltd., Qingdao 266431, China.
  • 6 Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266003, China.
  • 7 Department of Tumor Radiotherapy, Air Force Medical Center, PLA, Beijing 100142, China. Electronic address: xiaochuan8668@126.com.
  • 8 Department of Pharmaceutics, School of Pharmacy, Qingdao University, Qingdao 266021, China. Electronic address: sunyong@qdu.edu.cn.
PMID: 38781741 DOI: 10.1016/j.archoralbio.2024.106003
Abstract

Objective: This study aimed to investigate the expression of Serine Protease Inhibitor kazal type 1 (SPINK1) and its carcinogenic effect in oral tongue squamous cell carcinoma (OTSCC).

Design: Initially, bioinformatics analysis was conducted using data from The Cancer Genome Atlas and Gene Expression Omnibus to compare SPINK1 mRNA expression between malignant and adjacent tissues. Subsequently, the impact of differential expression on survival and Other clinical variables was examined. Additionally, histology microarray analysis was performed to assess SPINK1 protein expression in 35 cases of malignant and adjacent tissues. Finally, alterations in SPINK1 expression were evaluated to determine its biological phenotypes in OTSCC, including proliferation, Apoptosis, invasion, and metastasis.

Results: OTSCC tissues exhibit higher levels of SPINK1 compared to surrounding cancerous tissues. Notably, increased SPINK1 expression correlates with the pathological N stage and independently predicts overall survival among patients with OTSCC.

Conclusion: Suppression of SPINK1 inhibited OTSCC cell proliferation, invasion, and motility while promoting Apoptosis. These findings suggest that SPINK1 may serve as a prognostic biomarker as well as a potential therapeutic target for managing OTSCC.

Keywords

Biomarkers; Kazal Pancreatic; Prognosis; Tongue Neoplasms; Trypsin Inhibitor.

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