2. Academic Validation
  3. Tremella aurantialba polysaccharides alleviate ulcerative colitis in mice by improving intestinal barrier via modulating gut microbiota and inhibiting ferroptosis

Tremella aurantialba polysaccharides alleviate ulcerative colitis in mice by improving intestinal barrier via modulating gut microbiota and inhibiting ferroptosis

  • Int J Biol Macromol. 2024 Sep 20;281(Pt 4):135835. doi: 10.1016/j.ijbiomac.2024.135835.
Gong Peng 1,  Sisi Wang 1,  Hansi Zhang 2,  Fang Xie 1,  Li Jiao 3,  Ye Yuan 4,  Cheng Ma 5,  Hui Wu 6,  Zhaoli Meng 7
Affiliations

Affiliations

  • 1 Laboratory of Tumor Immunology, The first Hospital of Jilin University, Changchun 130021, China.
  • 2 College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
  • 3 Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming 650000, China.
  • 4 Department of Laboratory Medicine, Lequn Branch, The First Hospital of Jilin University, Changchun 130031, China.
  • 5 Jilin Yatai Biopharmaceutical Co., Ltd., Changchun 130032, China.
  • 6 Department of Neonatology, Children's Medical Center, First Hospital of Jilin University, Changchun 130021, China.
  • 7 Department of Neonatology, Children's Medical Center, First Hospital of Jilin University, Changchun 130021, China; Laboratory of Tumor Immunology, The first Hospital of Jilin University, Changchun 130021, China. Electronic address: mengzl@jlu.edu.cn.
PMID: 39306158 DOI: 10.1016/j.ijbiomac.2024.135835
Abstract

We aimed to investigate the effect of a polysaccharide from Tremella aurantialba on ulcerative colitis (UC), which targets Ferroptosis in epithelial cells. TA 2-1 (127 kDa) was isolated from T. aurantialba and consisted of Man, Xyl, GlcA, Glc, Fuc and Rha with a molar ratio of 59.2: 23.2: 13.9: 1.6: 1.7: 0.4, exhibited a 1, 3-Man structure with branch chains of T-Xylp, 1,3-Xylp, 1,4-GlcAp, and T-Manp at its O-2 position. TA 2-1 (100 μg/mL) inhibited the cell viability of Ferroptosis (19.8 %) in RLS3-induced Caco-2 cells and significantly ameliorated symptoms in the colons of mice with dextran sodium sulfate (DSS)-induced UC. TA 2-1 remarkably repaired the intestinal barrier by upregulating claudin-1 and zonula occludens-1 levels. Further analysis found TA 2-1 significantly suppressed lipid peroxidation by regulating ferroptosis-related proteins in UC mice, suggesting that its protective effects are partially mediated by inhibiting Ferroptosis. Further analysis of the gut microbiota and fecal microbiota transplantation revealed TA 2-1 might relieve UC symptoms or inhibit Ferroptosis by modulating the gut microbiota's composition or metabolites. Results suggest the protective effects of TA 2-1 on the intestinal barrier by inhibiting Ferroptosis of epithelial cells, at least by regulating the gut microbiota, highlighting the potential of TA 2-1 in UC treatment.

Keywords

Ferroptosis; Tremella aurantialba polysaccharides; Ulcerative colitis.

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