2. Academic Validation
  3. San Huang Xiao Yan recipe promoted wound healing in diabetic ulcer mice by inhibiting Th17 cell differentiation

San Huang Xiao Yan recipe promoted wound healing in diabetic ulcer mice by inhibiting Th17 cell differentiation

  • J Ethnopharmacol. 2025 Feb 11:341:119243. doi: 10.1016/j.jep.2024.119243.
Jie Deng 1,  Wanwan Gan 2,  Can Hu 1,  Zhe Liu 1,  Nan Chen 3,  Chenglin Jia 1,  Minlu Ding 1,  Jiaqi Zou 2,  Tongkai Cai 4,  Jiacheng Li 1,  Yicheng Xu 1,  Jian Chen 1,  Chao Ma 1,  Hao Yin 1,  Zhihui Zhang 5,  Haikun Wang 6,  Yongbing Cao 7
Affiliations

Affiliations

  • 1 Institute of Vascular Disease, Shanghai TCM-Integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200082, China.
  • 2 Institute of Vascular Disease, Shanghai TCM-Integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200082, China; Department of Physiology and Pharmacology, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, 210009, China.
  • 3 Institute of Vascular Disease, Shanghai TCM-Integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200082, China; The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Zhejiang, 310006, China.
  • 4 Shanghai Diacart Biomedical Science and Technology Limited Company, Shanghai, 201203, China.
  • 5 Institute of Vascular Disease, Shanghai TCM-Integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200082, China. Electronic address: 18715112312@163.com.
  • 6 State Key Laboratory of Cardiovascular Diseases, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200120, China. Electronic address: hkwang@tongji.edu.cn.
  • 7 Institute of Vascular Disease, Shanghai TCM-Integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200082, China. Electronic address: ybcao@vip.sina.com.
PMID: 39722327 DOI: 10.1016/j.jep.2024.119243
Abstract

Ethnopharmacological relevance: Diabetic ulcer is a serious diabetes complication and a primary reason for amputations. For many years, the San Huang Xiao Yan (SHXY) recipe has served as a conventional remedy for these ulcers, effectively reducing inflammatory factors and exhibiting considerable therapeutic efficacy. However, the precise mechanism remains incompletely understood.

Aim of the study: To explore the efficacy and mechanisms of SHXY and its active ingredients in treating diabetic ulcer.

Materials and methods: A diabetic ulcer mouse model was established using C57BL/6J mice on a high-fat diet, followed by streptozotocin injection and skin damage. We investigated the bioactive compounds, key targets, and pharmacological mechanisms of SHXY in addressing diabetic ulcers through network pharmacology, molecular docking, both in vitro and in vivo validation experiments.

Results: One week after intragastric administration, SHXY can reduce inflammation and edema, increase collagen synthesis, and reduce the expression of RORγT and IL-17A without affecting Treg cells. In vitro, SHXY-containing serum inhibited the differentiation of Th17 cells but did not affect Treg and Th1 cells. Network pharmacology found that SHXY acts through inflammatory pathways, including TNF, IL-17, Th17 cell differentiation, HIF-1, and PI3K-Akt.

Conclusions: SHXY and its candidate enhance healing in diabetic ulcers by modulating CD4+ T cells, particularly by inhibiting Th17 cell differentiation.

Keywords

Baicalein; CD4(+)T cell; Diabetic ulcer; Th17 cell differentiation; Wound healing.

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