Melflufen hydrochloride (Synonyms: Melphalan flufenamide hydrochloride)

Name: Melflufen hydrochloride
Brand: MedChemExpress
SKU: HY-105019A
Rating: 4.5 (1 reviews)

Cat. No.: HY-105019A Purity: 98.73%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Melflufen (Melphalan flufenamide) hydrochloride, a dipeptide proagent of Melphalan, is an alkylating agent. Melflufen hydrochloride shows antitumor activity against multiple myeloma (MM) cells and inhibits angiogenesis. Melflufen hydrochloride induces irreversible DNA damage and cytotoxicity in MM cells.

For research use only. We do not sell to patients.

Melflufen hydrochloride Chemical Structure

CAS No. : 380449-54-7

Size	Price	Stock	Quantity
5 mg	USD 420	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 700	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1900	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2900	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Melflufen hydrochloride:

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1 Publications Citing Use of MCE Melflufen hydrochloride

•Cell Death Dis. 2022 Feb 10;13(2):136. [Abstract]

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Melflufen (Melphalan flufenamide) hydrochloride (0.5-10 μM; 24 hours) decreases viability of MM.1S, INA-6, RPMI-8226, MM.1R, Dox-40, ARP-1, and ANBL-6 cells in a concentration-dependent manner^[1].
Melflufen hydrochloride induces apoptosis in MM.1S cells^[1].
Melflufen hydrochloride also is a potent activator of exosome secretion^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Multiple myeloma cells: MM.1S, INA-6, RPMI-8226, MM.1R, Dox-40, ARP-1, ANBL-6 cells
Concentration:	0.5, 1, 3, 5, 10 μM
Incubation Time:	24 hours
Result:	A significant concentration-dependent decrease in viability of all cell lines was observed.

In Vivo

Melflufen (Melphalan flufenamide) hydrochloride (3 mg/kg; i.v.; twice-weekly for two weeks) shows anti-MM activity in xenograft mouse model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	CB-17 SCID mice (human plasmacytoma MM.1S xenograft mouse model)^[1]
Dosage:	3 mg/kg
Administration:	1.v.; twice-weekly for two weeks
Result:	Significantly inhibited MM tumor growth and prolonged survival of mice.

Clinical Trial

Molecular Weight

534.88

Formula

C₂₄H₃₁Cl₃FN₃O₃

CAS No.

380449-54-7

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(OCC)[C@H](CC1=CC=C(F)C=C1)NC([C@H](CC2=CC=C(N(CCCl)CCCl)C=C2)N)=O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (62.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (1.87 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8696 mL	9.3479 mL	18.6958 mL
5 mM	0.3739 mL	1.8696 mL	3.7392 mL
10 mM	0.1870 mL	0.9348 mL	1.8696 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 1 mg/mL (1.87 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.73%

Select Batch:

Data Sheet (274 KB) SDS (584 KB)

COA (248 KB) LCMS (99 KB)

Handling Instructions (2659 KB)

References

[1]. Chauhan D, et al. In vitro and in vivo antitumor activity of a novel alkylating agent, melphalan-flufenamide, against multiple myeloma cells. Clin Cancer Res. 2013;19(11):3019-3031. [Content Brief]

[2]. Ray A, et al. A novel alkylating agent Melflufen induces irreversible DNA damage and cytotoxicity in multiple myeloma cells. Br J Haematol. 2016;174(3):397-409. [Content Brief]

[3]. McAndrews KM, et, al. Mechanisms associated with biogenesis of exosomes in cancer. Mol Cancer. 2019 Mar 30;18(1):52. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.8696 mL	9.3479 mL	18.6958 mL	46.7395 mL
DMSO	5 mM	0.3739 mL	1.8696 mL	3.7392 mL	9.3479 mL
	10 mM	0.1870 mL	0.9348 mL	1.8696 mL	4.6739 mL
	15 mM	0.1246 mL	0.6232 mL	1.2464 mL	3.1160 mL
	20 mM	0.0935 mL	0.4674 mL	0.9348 mL	2.3370 mL
	25 mM	0.0748 mL	0.3739 mL	0.7478 mL	1.8696 mL
	30 mM	0.0623 mL	0.3116 mL	0.6232 mL	1.5580 mL
	40 mM	0.0467 mL	0.2337 mL	0.4674 mL	1.1685 mL
	50 mM	0.0374 mL	0.1870 mL	0.3739 mL	0.9348 mL
	60 mM	0.0312 mL	0.1558 mL	0.3116 mL	0.7790 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Melflufen hydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Melflufen380449-54-7Melphalan flufenamideDNA Alkylator/CrosslinkerApoptosismultiplemyelomasurvivalMM.1ScellsDNAdamageangiogenesisgrowthInhibitorinhibitorinhibit

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Melflufen hydrochloride (Synonyms: Melphalan flufenamide hydrochloride)

Customer Review

Other Forms of Melflufen hydrochloride:

Melflufen hydrochloride Related Antibodies

1 Publications Citing Use of MCE Melflufen hydrochloride

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Melflufen hydrochloride Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Melflufen hydrochloride Related Classifications

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