Methyl L-histidinate dihydrochloride

Cat. No.: HY-Y0754 Purity: ≥97.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Methyl L-histidinate dihydrochloride is a histidine derivative.

For research use only. We do not sell to patients.

Methyl L-histidinate dihydrochloride Chemical Structure

CAS No. : 7389-87-9

Size	Price	Stock
100 g	USD 45	Ask For Quote & Lead Time
500 g	USD 175	Ask For Quote & Lead Time

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This product is a controlled substance and not for sale in your territory.

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Purity & Documentation
References
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Description

Methyl L-histidinate dihydrochloride is a histidine derivative^[1].

In Vitro

Amino acids and amino acid derivatives have been commercially used as ergogenic supplements. They influence the secretion of anabolic hormones, supply of fuel during exercise, mental performance during stress related tasks and prevent exercise induced muscle damage. They are recognized to be beneficial as ergogenic dietary substances^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

242.10

Formula

C₇H₁₃Cl₂N₃O₂

CAS No.

7389-87-9

Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (413.05 mM; Need ultrasonic)

DMSO : 7.69 mg/mL (31.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1305 mL	20.6526 mL	41.3052 mL
5 mM	0.8261 mL	4.1305 mL	8.2610 mL
10 mM	0.4131 mL	2.0653 mL	4.1305 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.77 mg/mL (3.18 mM); Clear solution

This protocol yields a clear solution of ≥ 0.77 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 0.77 mg/mL (3.18 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.77 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.77 mg/mL (3.18 mM); Clear solution

This protocol yields a clear solution of ≥ 0.77 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥97.0%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (252 KB) HNMR (118 KB)

Handling Instructions (2659 KB)

References

[1]. Luckose F, et al. Effects of amino acid derivatives on physical, mental, and physiological activities. Crit Rev Food Sci Nutr. 2015;55(13):1793-1144. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.1305 mL	20.6526 mL	41.3052 mL	103.2631 mL
	5 mM	0.8261 mL	4.1305 mL	8.2610 mL	20.6526 mL
	10 mM	0.4131 mL	2.0653 mL	4.1305 mL	10.3263 mL
	15 mM	0.2754 mL	1.3768 mL	2.7537 mL	6.8842 mL
	20 mM	0.2065 mL	1.0326 mL	2.0653 mL	5.1632 mL
	25 mM	0.1652 mL	0.8261 mL	1.6522 mL	4.1305 mL
	30 mM	0.1377 mL	0.6884 mL	1.3768 mL	3.4421 mL
H₂O	40 mM	0.1033 mL	0.5163 mL	1.0326 mL	2.5816 mL
	50 mM	0.0826 mL	0.4131 mL	0.8261 mL	2.0653 mL
	60 mM	0.0688 mL	0.3442 mL	0.6884 mL	1.7211 mL
	80 mM	0.0516 mL	0.2582 mL	0.5163 mL	1.2908 mL
	100 mM	0.0413 mL	0.2065 mL	0.4131 mL	1.0326 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.