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  4. Methyl Orange

Methyl Orange is a soluble azo dye commonly used as an acid-base indicator and for staining cells and tissue sections, as well as for dyeing textiles. Methyl Orange appears red at a pH of 3.1 and changes to bright yellow as the pH increases to 4.4. Methyl Orange (500 mg/L) exhibits cytotoxicity and can cause DNA damage.

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Methyl Orange Chemical Structure

Methyl Orange Chemical Structure

CAS No. : 547-58-0

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Other Forms of Methyl Orange:

Methyl Orange Related Antibodies

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Description

Methyl Orange is a soluble azo dye commonly used as an acid-base indicator and for staining cells and tissue sections, as well as for dyeing textiles. Methyl Orange appears red at a pH of 3.1 and changes to bright yellow as the pH increases to 4.4. Methyl Orange (500 mg/L) exhibits cytotoxicity and can cause DNA damage[1][2][3].

In Vivo

1. Acid-Base Indicator[2]
Methyl Orange exhibits a distinct color change at different pH values:
pH < 3.1: Red
pH 3.1 - 4.4: Orange
pH > 4.4: Bright Yellow
2. Methyl Orange Vivo Staining Effects and Toxicity Studies[3]
2.1 Preparation of Working Solution
Dissolve Methyl Orange in Hoagland nutrient solution to a concentration of 2.0 mmol/L.
2.2 Vivo Staining
(1).Select healthy, uniformly sized, well-rooted, and pest-free Spider Plant cuttings and transplant them into Hoagland nutrient solution containing 2.0 mmol/L Methyl Orange at pH 6.5. Change the nutrient solution every 3 days.
(2).On days 20, 40, and 60 of treatment, randomly select 10 mature, healthy, pest-free leaves from each plant and measure the leaf color parameters in the middle of the leaf surface using an NC-9801 handheld colorimeter.
(3).On day 60 of treatment, measure the biomass and root-to-shoot ratio, where root-to-shoot ratio = (fresh weight of underground part / fresh weight of above-ground part) × 100%.
(4).Starting from day 10 of treatment, measure leaf nutrient content every 10 days, including soluble sugars, starch, soluble proteins, total nitrogen, chlorophyll, and carotenoids.
1.3 Data Analysis Perform significance analysis using SPSS 22.0 software to determine the staining effect of Methyl Orange on Spider Plants and its impact on their biological activity.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

327.33

Formula

C14H14N3NaO3S
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O=S(C1=CC=C(C=C1)/N=N/C2=CC=C(C=C2)N(C)C)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (190.94 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0550 mL 15.2751 mL 30.5502 mL
5 mM 0.6110 mL 3.0550 mL 6.1100 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (6.35 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (6.35 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0550 mL 15.2751 mL 30.5502 mL 76.3755 mL
5 mM 0.6110 mL 3.0550 mL 6.1100 mL 15.2751 mL
10 mM 0.3055 mL 1.5275 mL 3.0550 mL 7.6376 mL
15 mM 0.2037 mL 1.0183 mL 2.0367 mL 5.0917 mL
20 mM 0.1528 mL 0.7638 mL 1.5275 mL 3.8188 mL
25 mM 0.1222 mL 0.6110 mL 1.2220 mL 3.0550 mL
30 mM 0.1018 mL 0.5092 mL 1.0183 mL 2.5459 mL
40 mM 0.0764 mL 0.3819 mL 0.7638 mL 1.9094 mL
50 mM 0.0611 mL 0.3055 mL 0.6110 mL 1.5275 mL
60 mM 0.0509 mL 0.2546 mL 0.5092 mL 1.2729 mL
80 mM 0.0382 mL 0.1909 mL 0.3819 mL 0.9547 mL
100 mM 0.0306 mL 0.1528 mL 0.3055 mL 0.7638 mL
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Methyl Orange Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methyl Orange547-58-0Fluorescent Dyeazo dyeorganic contaminantInhibitorinhibitorinhibit

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