METTL3 activator-1

Name: METTL3 activator-1
Brand: MedChemExpress
SKU: HY-W037893
Rating: 4.5 (1 reviews)

Cat. No.: HY-W037893 Purity: 99.38%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

METTL3 activator-1 (2-Piperazinecarboxylic acid methyl ester dihydrochloride) is a Mettl3 activator that can promote the expression of Mettl3. METTL3 activator-1 can be used for the study of liver fibrosis.

For research use only. We do not sell to patients.

METTL3 activator-1 Chemical Structure

CAS No. : 122323-88-0

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Molecular Weight

217.09

Formula

C₆H₁₄Cl₂N₂O₂

CAS No.

122323-88-0

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1NCCNC1)OC.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature, keep dry and cool

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

H₂O : 250 mg/mL (1151.60 mM; Need ultrasonic)

DMSO : 100 mg/mL (460.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.6064 mL	23.0319 mL	46.0638 mL
5 mM	0.9213 mL	4.6064 mL	9.2128 mL
10 mM	0.4606 mL	2.3032 mL	4.6064 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (23.03 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (23.03 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (23.03 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.38%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (250 KB) HNMR (271 KB) RP-HPLC (247 KB)

Handling Instructions (2659 KB)

References

[1]. Xinlong Yan, et al. Application of compound in preparation of drugs for improving or treating liver injury and/or liver fibrosis. CN117731668A.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.6064 mL	23.0319 mL	46.0638 mL	115.1596 mL
	5 mM	0.9213 mL	4.6064 mL	9.2128 mL	23.0319 mL
	10 mM	0.4606 mL	2.3032 mL	4.6064 mL	11.5160 mL
	15 mM	0.3071 mL	1.5355 mL	3.0709 mL	7.6773 mL
	20 mM	0.2303 mL	1.1516 mL	2.3032 mL	5.7580 mL
	25 mM	0.1843 mL	0.9213 mL	1.8426 mL	4.6064 mL
	30 mM	0.1535 mL	0.7677 mL	1.5355 mL	3.8387 mL
	40 mM	0.1152 mL	0.5758 mL	1.1516 mL	2.8790 mL
	50 mM	0.0921 mL	0.4606 mL	0.9213 mL	2.3032 mL
	60 mM	0.0768 mL	0.3839 mL	0.7677 mL	1.9193 mL
	80 mM	0.0576 mL	0.2879 mL	0.5758 mL	1.4395 mL
	100 mM	0.0461 mL	0.2303 mL	0.4606 mL	1.1516 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.