2. Apoptosis
  3. Apoptosis
  4. Misetionamide

Misetionamide  (Synonyms: GP-2250)

Cat. No.: HY-147280 Purity: 99.49%
SDS COA Handling Instructions

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Misetionamide is an orally oxathiazin-like compound. Misetionamide is a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) inhibitor with antineoplastic activity. Misetionamide can be used for the research of cancer.

For research use only. We do not sell to patients.

Misetionamide Chemical Structure

Misetionamide Chemical Structure

CAS No. : 856785-75-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 638 In-stock
Solution
10 mM * 1 mL in DMSO USD 638 In-stock
Solid
1 mg USD 232 In-stock
5 mg USD 580 In-stock
10 mg USD 940 In-stock
25 mg USD 1780 In-stock
50 mg USD 2850 In-stock
100 mg USD 4550 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Misetionamide Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Misetionamide is an orally oxathiazin-like compound. Misetionamide is a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) inhibitor with antineoplastic activity. Misetionamide can be used for the research of cancer[1].

In Vitro

Misetionamide (OTD) (0-2000 μM; 0-72 h) induces cell death in TNBC cells[2].
Misetionamide (OTD) (0-2000 μM; 12 h) inhibits TNBC cells proliferation[2].
Misetionamide (OTD) (0-1000 μM; 18 h) induces necrosis and apoptosis in TNBC cells[2].
Misetionamide (OTD) (250-1000 μM; 18 h) induces cell death by a ROS-dependent mechanism in TNBC cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Misetionamide Related Antibodies

Cell Viability Assay[2]

Cell Line: TNBC cells
Concentration: 0, 100, 200, 500, 1000 and 2000 μM
Incubation Time: 0, 24, 48 and 72 hours
Result: Induced cell death in TNBC cells dose- and time-dependently.

Cell Proliferation Assay[2]

Cell Line: TNBC cells
Concentration: 0, 100, 200, 500, 1000 and 2000 μM
Incubation Time: 12 hours
Result: Inhibited TNBC cell proliferation dose-dependently.

Cell Viability Assay[2]

Cell Line: TNBC cells
Concentration: 250, 500, 750, 1000 μM
Incubation Time: 24 hours
Result: Induced TNBC cell death mainly dependent on ROS generation.

Apoptosis Analysis[2]

Cell Line: TNBC cells
Concentration: 0, 100, 250, 750, 1000 μM
Incubation Time: 18 hours
Result: Induced necrosis and apoptosis in TNBC cells dose-dependently and induced cell apoptosis is a underlying mechanism to kill TNBC cells.
Molecular Weight

137.16

Formula

C3H7NO3S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S1(NCOCC1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (729.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.2908 mL 36.4538 mL 72.9076 mL
5 mM 1.4582 mL 7.2908 mL 14.5815 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.49%

SDS (251 KB)

COA (254 KB) HNMR (193 KB) RP-HPLC (284 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 7.2908 mL 36.4538 mL 72.9076 mL 182.2689 mL
5 mM 1.4582 mL 7.2908 mL 14.5815 mL 36.4538 mL
10 mM 0.7291 mL 3.6454 mL 7.2908 mL 18.2269 mL
15 mM 0.4861 mL 2.4303 mL 4.8605 mL 12.1513 mL
20 mM 0.3645 mL 1.8227 mL 3.6454 mL 9.1134 mL
25 mM 0.2916 mL 1.4582 mL 2.9163 mL 7.2908 mL
30 mM 0.2430 mL 1.2151 mL 2.4303 mL 6.0756 mL
40 mM 0.1823 mL 0.9113 mL 1.8227 mL 4.5567 mL
50 mM 0.1458 mL 0.7291 mL 1.4582 mL 3.6454 mL
60 mM 0.1215 mL 0.6076 mL 1.2151 mL 3.0378 mL
80 mM 0.0911 mL 0.4557 mL 0.9113 mL 2.2784 mL
100 mM 0.0729 mL 0.3645 mL 0.7291 mL 1.8227 mL
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Misetionamide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Misetionamide856785-75-6GP-2250GP2250GP 2250Apoptosisoxathiazine-likeantagonistGAPDHATPROSInhibitorinhibitorinhibit

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