2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. TRP Channel
  4. ML-SI1

ML-SI1, a racemic mixture of diastereomers, is a TRPML inhibitor with an IC50 value of 15 μM for TRPML1.

For research use only. We do not sell to patients.

ML-SI1 Chemical Structure

ML-SI1 Chemical Structure

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 385 In-stock
Solution
10 mM * 1 mL in DMSO USD 385 In-stock
Solid
1 mg USD 140 In-stock
5 mg USD 350 In-stock
10 mg USD 580 In-stock
25 mg USD 1100 In-stock
50 mg USD 1700 In-stock
100 mg USD 2500 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 7 publication(s) in Google Scholar

ML-SI1 Related Antibodies

Top Publications Citing Use of Products

    ML-SI1 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Apr 20;257:114942.  [Abstract]

    ML-SI1 (1.25 μM; 24 h) significantly reduces the AFB1-induced expression of LC3, SQSTM1, Cleaved-Caspase3 and TRPML1 in IPEC-J2 cells.

    ML-SI1 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Apr 20;257:114942.  [Abstract]

    ML-SI1 (1.25 μM; 24 h) significantly reduces the AFB1-induced number of autophagosomes in IPEC-J2 cells.

    View All TRP Channel Isoform Specific Products:

    View All Isoforms
    TRPC TRPM TRPV TRPA TRPML

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    ML-SI1, a racemic mixture of diastereomers, is a TRPML inhibitor with an IC50 value of 15 μM for TRPML1[1].

    IC50 & Target

    IC50: 15 μM (TRPML1)[1]
    In Vitro

    ML-SI1 is an inseparable racemic mixture of cis-/trans-isomers (55:45) in a short synthetic sequence and its inhibitory activity on hTRPML1 (and a weak effect on TRPML2) after activation with ML-SA1. Fura-2 based single cell calcium imaging experiments confirmed that the synthesized racemic ML-SI1 (10 μM) has an inhibitory effect on hTRPML1[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ML-SI1 Related Antibodies
    Molecular Weight

    449.37

    Formula

    C23H26Cl2N2O3
    Appearance

    Solid

    Color

    Off-white to yellow

    SMILES

    O=C(N1C2=CC=C(C=C2C(C1C)CCN3CCOCC3)OC)C4=C(Cl)C(Cl)=CC=C4.[Mixture of diastereomers]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (222.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2253 mL 11.1267 mL 22.2534 mL
    5 mM 0.4451 mL 2.2253 mL 4.4507 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (5.56 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (5.56 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.85%

    COA (258 KB) LCMS (97 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2253 mL 11.1267 mL 22.2534 mL 55.6334 mL
    5 mM 0.4451 mL 2.2253 mL 4.4507 mL 11.1267 mL
    10 mM 0.2225 mL 1.1127 mL 2.2253 mL 5.5633 mL
    15 mM 0.1484 mL 0.7418 mL 1.4836 mL 3.7089 mL
    20 mM 0.1113 mL 0.5563 mL 1.1127 mL 2.7817 mL
    25 mM 0.0890 mL 0.4451 mL 0.8901 mL 2.2253 mL
    30 mM 0.0742 mL 0.3709 mL 0.7418 mL 1.8544 mL
    40 mM 0.0556 mL 0.2782 mL 0.5563 mL 1.3908 mL
    50 mM 0.0445 mL 0.2225 mL 0.4451 mL 1.1127 mL
    60 mM 0.0371 mL 0.1854 mL 0.3709 mL 0.9272 mL
    80 mM 0.0278 mL 0.1391 mL 0.2782 mL 0.6954 mL
    100 mM 0.0223 mL 0.1113 mL 0.2225 mL 0.5563 mL
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    ML-SI1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ML-SI1TRP ChannelTransient receptor potential channelsTRPML1diastereomersInhibitorinhibitorinhibit

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