MM-401 TFA

Name: MM-401 TFA
Brand: MedChemExpress
SKU: HY-19554A
Rating: 4.5 (1 reviews)

Cat. No.: HY-19554A Purity: 99.62%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

MM-401 (TFA) is a MLL1 H3K4 methyltransferase inhibitor. MM-401 inhibits MLL1 activity (IC₅₀ = 0.32 µM) by blocking MLL1-WDR5 interaction. MM-401 can induce cell cycle arrest, apoptosis and differentiation. MM-401 can be used for the research of MLL leukemia.

For research use only. We do not sell to patients.

MM-401 TFA Chemical Structure

CAS No. : 1442106-11-7

Size	Price	Stock	Quantity
1 mg	USD 490	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 1120	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1800	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of MM-401 TFA:

MM-401 Get quote

1 Publications Citing Use of MCE MM-401 TFA

•Nat Commun. 2023 Sep 14;14(1):5685. [Abstract]

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View All Histone Methyltransferase Isoform Specific Products:

View All Isoforms

EZH1 EZH2 DOT1L SMYD2 SMYD3 SUV39H2/KMT1B EHMT2/G9a/KMT1C EHMT1/GLP/KMT1D SETDB1/KMT2G SETD2/KMT3A NSD2/MMSET/WHSC1 SETD7/KMT7 SETD8/KMT5A PRMT1 PRMT3 PRMT4 PRMT5 PRMT6 PRMT7 PRMT8

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Ki: < 1 nM (WDR5); IC50: 0.9 nM (WDR5-MLL1 interaction), 0.32 µM (MLL1)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	> 100 μM Compound: 2	Cytotoxicity against human HL60 cells lacking MLL translocation assessed as cell growth inhibition after 4 days by WST8 assay Cytotoxicity against human HL60 cells lacking MLL translocation assessed as cell growth inhibition after 4 days by WST8 assay	[PMID: 28603984]
MOLM-13	IC₅₀	18.4 μM Compound: 2	Cytotoxicity against human MOLM13 cells harboring MLL-AF9 fusion protein assessed as cell growth inhibition after 4 days by WST8 assay Cytotoxicity against human MOLM13 cells harboring MLL-AF9 fusion protein assessed as cell growth inhibition after 4 days by WST8 assay	[PMID: 28603984]
MV4-11	IC₅₀	9.8 μM Compound: 2	Cytotoxicity against human MV4-11 cells harboring MLL-AF4 fusion protein assessed as cell growth inhibition after 4 days by WST8 assay Cytotoxicity against human MV4-11 cells harboring MLL-AF4 fusion protein assessed as cell growth inhibition after 4 days by WST8 assay	[PMID: 28603984]

In Vitro

MM-401 maintains high binding affinity to WDR5 with a K_i value of < 1 nM and disrupts WDR5-MLL1 interaction with an IC₅₀ value of 0.9 nM^[1].
MM-401 is able to specifically inhibit MLL1 activity (IC₅₀ value of 0.32µM) by blocking MLL1-WDR5 interaction and thus the complex assembly^[1].
MM-401 (20 μM; 48 h) specifically inhibits MLL1-dependent H3K4 methylation in cells^[1].
MM-401 induces similar changes in MLL-AF9 transcriptome as the MLL1 deletion^[1].
MM-401 (10, 20, 40 μM; 48 h) specifically inhibits growth of MLL leukemia cells by inducing cell cycle arrest, apoptosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:	Murine MLL-AF9 and Hoxa9/Meis1 cells
Concentration:	10, 20, 40 μM
Incubation Time:	48 h
Result:	Specifically induced apoptosis of MLL-AF9 cells.

Cell Cycle Analysis^[1]

Cell Line:	Murine MLL-AF9 and Hoxa9/Meis1 cells
Concentration:	10, 20, 40 μM
Incubation Time:	48 h
Result:	Induced prominent G1/S arrest in MLL-AF9 cells in a concentration dependent manner.

RT-PCR^[1]

Cell Line:	MLL-AF9 cells
Concentration:	20 μM
Incubation Time:	48 h
Result:	Significantly decreased H3K4me, expression of 5 Hox A genes, especially Hoxa9 and Hoxa10.

Molecular Weight

700.75

Formula

C₃₁H₄₇F₃N₈O₇

CAS No.

1442106-11-7

Appearance

Solid

Color

White to light yellow

SMILES

C[C@@](C(N[C@@H](CCCNC(N)=N)C(N[C@H](C(N[C@@]1(C2=CC=CC=C2)[H])=O)CC)=O)=O)(NC(C(C)C)=O)CCCCNC1=O.O=C(O)C(F)(F)F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (142.70 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4270 mL	7.1352 mL	14.2704 mL
5 mM	0.2854 mL	1.4270 mL	2.8541 mL
10 mM	0.1427 mL	0.7135 mL	1.4270 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.62%

Select Batch:

Data Sheet (277 KB) SDS (251 KB)

COA (254 KB) RP-HPLC (244 KB) LCMS (259 KB)

Handling Instructions (2659 KB)

References

[1]. Fang Cao, et al. Targeting MLL1 H3K4 methyltransferase activity in mixed-lineage leukemia. Mol Cell. 2014 Jan 23;53(2):247-61. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4270 mL	7.1352 mL	14.2704 mL	35.6761 mL
	5 mM	0.2854 mL	1.4270 mL	2.8541 mL	7.1352 mL
	10 mM	0.1427 mL	0.7135 mL	1.4270 mL	3.5676 mL
	15 mM	0.0951 mL	0.4757 mL	0.9514 mL	2.3784 mL
	20 mM	0.0714 mL	0.3568 mL	0.7135 mL	1.7838 mL
	25 mM	0.0571 mL	0.2854 mL	0.5708 mL	1.4270 mL
	30 mM	0.0476 mL	0.2378 mL	0.4757 mL	1.1892 mL
	40 mM	0.0357 mL	0.1784 mL	0.3568 mL	0.8919 mL
	50 mM	0.0285 mL	0.1427 mL	0.2854 mL	0.7135 mL
	60 mM	0.0238 mL	0.1189 mL	0.2378 mL	0.5946 mL
	80 mM	0.0178 mL	0.0892 mL	0.1784 mL	0.4460 mL
	100 mM	0.0143 mL	0.0714 mL	0.1427 mL	0.3568 mL

MM-401 TFA Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MM-4011442106-11-7MM401MM 401Histone MethyltransferaseApoptosisMLL1-WDR5 interactionMLL1histone methyltransferasesleukemiaH3K4cell cycleapoptosisdifferentiationInhibitorinhibitorinhibit

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MM-401 TFA

Customer Review

Other Forms of MM-401 TFA:

MM-401 TFA Related Antibodies

1 Publications Citing Use of MCE MM-401 TFA

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Histone Methyltransferase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

MM-401 TFA Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

MM-401 TFA Related Classifications

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