Myxothiazol

Name: Myxothiazol
Brand: MedChemExpress
SKU: HY-112177
Rating: 4.5 (1 reviews)

Cat. No.: HY-112177 Purity: ≥99.0%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Myxothiazol, an antifungal antibiotic, is a mitochondrial electron transport chain complex III (bc1 complex) inhibitor. Myxothiazol inhibits the growth of many yeasts and fungi at concentrations between 0.01 and 3 μg/ml.

For research use only. We do not sell to patients.

Myxothiazol Chemical Structure

CAS No. : 76706-55-3

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.01 ng/mL Compound: Myxothiazol	Cytotoxicity against human A549 cells after 72 hrs by SRB assay Cytotoxicity against human A549 cells after 72 hrs by SRB assay	[PMID: 10096868]
HCT-15	IC₅₀	0.03 ng/mL Compound: Myxothiazol	Cytotoxicity against human HCT-15 cells after 72 hrs by SRB assay Cytotoxicity against human HCT-15 cells after 72 hrs by SRB assay	[PMID: 10096868]
SK-MEL-2	IC₅₀	9.7 ng/mL Compound: Myxothiazol	Cytotoxicity against human SK-MEL-2 cells after 72 hrs by SRB assay Cytotoxicity against human SK-MEL-2 cells after 72 hrs by SRB assay	[PMID: 10096868]
SK-OV-3	IC₅₀	0.01 ng/mL Compound: Myxothiazol	Cytotoxicity against human SKOV3 cells after 72 hrs by SRB assay Cytotoxicity against human SKOV3 cells after 72 hrs by SRB assay	[PMID: 10096868]
XF498	IC₅₀	0.03 ng/mL Compound: Myxothiazol	Cytotoxicity against human XF498 cells after 72 hrs by SRB assay Cytotoxicity against human XF498 cells after 72 hrs by SRB assay	[PMID: 10096868]

In Vitro

Myxothiazol inhibits the growth of many yeasts and fungi at concentrations between 0.01 and 3 μg/ml^[2].
? Myxothiazol binds to the ubiquinol oxidation site Qo of complex III and blocks electron transfer from ubiquinol to cytochrome b and thus inhibits complex III activity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Myxothiazol (i.p.; 0.56 mg/kg; daily for 4 days)-induced complex III inhibition can be induced in mice for four days in a row without overt hepatotoxicity or lethality^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57Bl/J6 mice^[3]
Dosage:	0.56 mg/kg
Administration:	I.p.; 24 hours intervals for at most 4 times
Result:	A reversible complex III activity decrease to 50% of control value occurred at 2 h post-injection. At 74 h only minor histological changes in the liver were found, supercomplex formation was preserved and no significant changes in the expression of genes indicating hepatotoxicity or inflammation were found.

Molecular Weight

487.68

Formula

C₂₅H₃₃N₃O₃S₂

CAS No.

76706-55-3

Appearance

Solid

Color

White to off-white

SMILES

C[C@H](C1=NC(C2=NC(/C=C/[C@H](OC)[C@@H](C)/C(OC)=C\C(N)=O)=CS2)=CS1)/C=C/C=C/C(C)C

Structure Classification

Initial Source

Microorganisms

Myxococcus fulvus

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (102.53 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0505 mL	10.2526 mL	20.5052 mL
5 mM	0.4101 mL	2.0505 mL	4.1010 mL
10 mM	0.2051 mL	1.0253 mL	2.0505 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.56 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.53%

Select Batch:

Data Sheet (271 KB) SDS (420 KB)

COA (245 KB)

Handling Instructions (2659 KB)

References

[1]. Thierbach G, et al. Myxothiazol, a new antibiotic interfering with respiration. Antimicrob Agents Chemother. 1981;19(4):504-507. [Content Brief]

[2]. von Jagow G,et al. An inhibitor of mitochondrial respiration which binds to cytochrome b and displaces quinone from the iron-sulfur protein of the cytochrome bc1 complex. J Biol Chem. 1984;259(10):6318-6326. [Content Brief]

[3]. Davoudi M, et al. A mouse model of mitochondrial complex III dysfunction induced by myxothiazol. Biochem Biophys Res Commun. 2014;446(4):1079-1084. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0505 mL	10.2526 mL	20.5052 mL	51.2631 mL
	5 mM	0.4101 mL	2.0505 mL	4.1010 mL	10.2526 mL
	10 mM	0.2051 mL	1.0253 mL	2.0505 mL	5.1263 mL
	15 mM	0.1367 mL	0.6835 mL	1.3670 mL	3.4175 mL
	20 mM	0.1025 mL	0.5126 mL	1.0253 mL	2.5632 mL
	25 mM	0.0820 mL	0.4101 mL	0.8202 mL	2.0505 mL
	30 mM	0.0684 mL	0.3418 mL	0.6835 mL	1.7088 mL
	40 mM	0.0513 mL	0.2563 mL	0.5126 mL	1.2816 mL
	50 mM	0.0410 mL	0.2051 mL	0.4101 mL	1.0253 mL
	60 mM	0.0342 mL	0.1709 mL	0.3418 mL	0.8544 mL
	80 mM	0.0256 mL	0.1282 mL	0.2563 mL	0.6408 mL
	100 mM	0.0205 mL	0.1025 mL	0.2051 mL	0.5126 mL

Myxothiazol Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Myxothiazol76706-55-3FungalMitochondrial MetabolismAntibioticfungimitochondrialelectrontransportchainrespiratorytoxicityInhibitorinhibitorinhibit

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Myxothiazol

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Myxothiazol Related Antibodies

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Myxothiazol Related Antibodies

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Complete Stock Solution Preparation Table

Myxothiazol Related Classifications

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