MZ-101 (Synonyms: GYS1-IN-2)

Cat. No.: HY-157646 Purity: 98.98%: FAQs
Data Sheet SDS COA Handling Instructions

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MZ-101 (GYS1-IN-2) is an orally active, potent and selective small-molecule glycogen synthase 1 (GYS1) inhibitor with with an IC₅₀ value of 0.041 µM. MZ-101 reduces glycogen concentrations in cells and in mice. MZ-101 can used to study GYS1 -mediated Pompe disease and other glycogen storage diseases.

For research use only. We do not sell to patients.

MZ-101 Chemical Structure

CAS No. : 2839908-40-4

Size		Stock
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution		Check price and availability
Solution
10 mM * 1 mL in DMSO		Check price and availability
Solid
5 mg		Check price and availability
10 mg		Check price and availability

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This product is a controlled substance and not for sale in your territory.

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Description

In Vitro

MZ-101 (0.001-100 μM, 7 days) inhibits glycogen synthesis and reduces glycogen accumulation in fibroblasts expressing GYS1 ^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

MZ-101 (2-200 mg/kg, p.o., a single dose for 5 h) inhibits GYS1 but not GYS2 de novo glycogen synthesis and reduces glycogen combined with substrate reduction therapy in WT and Pompe mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57Bl6 mice between 7 and 10 weeks old and male Pompe mice between 10 and 32 weeks old^[2]
Dosage:	2-200 mg/kg
Administration:	p.o., a single dose for 5 h
Result:	Reduced incorporation of glucose into glycogen in skeletal and cardiac muscles of both WT and GAA KO mice in a dose-dependent manner.

Molecular Weight

449.52

Formula

C₂₅H₂₈FN₅O₂

CAS No.

2839908-40-4

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C([C@H]1N(C[C@@H](C1)F)C(CC2=CN=NN2)=O)N[C@@H](C3=CC=CC=C3)C4=CC=C(C=C4)C(C)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (222.46 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2246 mL	11.1230 mL	22.2460 mL
5 mM	0.4449 mL	2.2246 mL	4.4492 mL
10 mM	0.2225 mL	1.1123 mL	2.2246 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (11.12 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (11.12 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (11.12 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.98%

Select Batch:

Data Sheet (279 KB) SDS (251 KB)

COA (257 KB) HNMR (161 KB) RP-HPLC (153 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. David John Morgans, et al. Inhibitors of glycogen synthase 1 (gys1) and methods of use thereof. WO2022198196. 2022-09-22.

[2]. Ullman JC, et al. Small-molecule inhibition of glycogen synthase 1 for the treatment of Pompe disease and other glycogen storage disorders. Sci Transl Med. 2024 Jan 17;16(730):eadf1691. [Content Brief]

[3]. Gaspar RC, et al. Small molecule inhibition of glycogen synthase I reduces muscle glycogen content and improves biomarkers in a mouse model of Pompe disease. Am J Physiol Endocrinol Metab. 2024 Oct 1;327(4):E524-E532. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2246 mL	11.1230 mL	22.2460 mL	55.6149 mL
	5 mM	0.4449 mL	2.2246 mL	4.4492 mL	11.1230 mL
	10 mM	0.2225 mL	1.1123 mL	2.2246 mL	5.5615 mL
	15 mM	0.1483 mL	0.7415 mL	1.4831 mL	3.7077 mL
	20 mM	0.1112 mL	0.5561 mL	1.1123 mL	2.7807 mL
	25 mM	0.0890 mL	0.4449 mL	0.8898 mL	2.2246 mL
	30 mM	0.0742 mL	0.3708 mL	0.7415 mL	1.8538 mL
	40 mM	0.0556 mL	0.2781 mL	0.5561 mL	1.3904 mL
	50 mM	0.0445 mL	0.2225 mL	0.4449 mL	1.1123 mL
	60 mM	0.0371 mL	0.1854 mL	0.3708 mL	0.9269 mL
	80 mM	0.0278 mL	0.1390 mL	0.2781 mL	0.6952 mL
	100 mM	0.0222 mL	0.1112 mL	0.2225 mL	0.5561 mL

MZ-101 Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MZ-1012839908-40-4GYS1-IN-2MZ101MZ 101Othersorally activeGYS1glycogen concentrationsPompe diseaseInhibitorinhibitorinhibit

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