Nampt activator-3

Name: Nampt activator-3
Brand: MedChemExpress
SKU: HY-148948
Rating: 4.5 (1 reviews)

Cat. No.: HY-148948 Purity: 99.67%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

NAMPT activator-3, a NAT derivative, is a NAMPT activator with an EC₅₀ of 2.6 μM and a K_D of 132 nM. NAMPT activator-3 effectively protects cultured cells from FK866 (HY-50876)-mediated toxicity. NAMPT activator-3 exhibits strong neuroprotective efficacy in a chemotherapy-induced peripheral neuropathy (CIPN) mouse model without any overt toxicity.

For research use only. We do not sell to patients.

Nampt activator-3 Chemical Structure

CAS No. : 2790481-63-7

Size	Price	Stock	Quantity
5 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 700	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1100	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1650	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

NAMPT activator-3 (compound 72; 0.1, 0.3, 1, 3, 10 μM; 72 hours) exertes no side effect on the cell viability including osteosarcoma cells (U2OS), glioblastoma cells (T98G), neuroblastoma cells (SH-SY5Y) and liver carcinoma cells (HepG2)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

NAMPT activator-3 (compound 72) protects peripheral sensory neurons from Taxol (HY-B0015)-induced damage by enhancing NAD production^[1].
Pharmacokinetic Parameters of NAMPT activator-3 in mice^[1].

	IV (1 mg/kg)	IP (30 mg/kg)	PO (30 mg/kg)
T_max (h)			0.25
C_max (ng/mL)		1563	188
AUC_last (h∗ng/mL)	183	1333	388
T_1/2 (h)	0.18	1.05	1.34
CL (mL/min/kg)	90.4
V_ss (mL/kg)	1130
F (%)		24.2	7.05

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6J mice^[1]
Dosage:	1 or 3 mg/kg
Administration:	IP; daily for one week followed by Taxol (18.3 mg/kg; ip; on day 9, 11, and 13)
Result:	Showed a dose-dependent effect on elevation of paw withdrawal threshold. Largely restored the density of myelinated fibers relative to the vehicle control group. Significantly elevate NAD level in the sciatic nerves.

Molecular Weight

324.37

Formula

C₁₉H₂₀N₂O₃

CAS No.

2790481-63-7

Appearance

Solid

Color

White to off-white

SMILES

N#CC1=CC=CC(C(C)(C)C)=C1OCC(NC2=CC=C(C=C2)O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (308.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0829 mL	15.4145 mL	30.8290 mL
5 mM	0.6166 mL	3.0829 mL	6.1658 mL
10 mM	0.3083 mL	1.5414 mL	3.0829 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (7.71 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (7.71 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (7.71 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.67%

Select Batch:

Data Sheet (274 KB) SDS (251 KB)

COA (248 KB) HNMR (116 KB) RP-HPLC (245 KB)

Handling Instructions (2659 KB)

References

[1]. Leibo Wang, et al. Optimization of NAMPT activators to achieve in vivo neuroprotective efficacy. Eur J Med Chem. 2022 Jun 5;236:114260. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0829 mL	15.4145 mL	30.8290 mL	77.0725 mL
	5 mM	0.6166 mL	3.0829 mL	6.1658 mL	15.4145 mL
	10 mM	0.3083 mL	1.5414 mL	3.0829 mL	7.7072 mL
	15 mM	0.2055 mL	1.0276 mL	2.0553 mL	5.1382 mL
	20 mM	0.1541 mL	0.7707 mL	1.5414 mL	3.8536 mL
	25 mM	0.1233 mL	0.6166 mL	1.2332 mL	3.0829 mL
	30 mM	0.1028 mL	0.5138 mL	1.0276 mL	2.5691 mL
	40 mM	0.0771 mL	0.3854 mL	0.7707 mL	1.9268 mL
	50 mM	0.0617 mL	0.3083 mL	0.6166 mL	1.5414 mL
	60 mM	0.0514 mL	0.2569 mL	0.5138 mL	1.2845 mL
	80 mM	0.0385 mL	0.1927 mL	0.3854 mL	0.9634 mL
	100 mM	0.0308 mL	0.1541 mL	0.3083 mL	0.7707 mL