Naphthazarin (Synonyms: DHNQ; 5,8-Dihydroxy-1,4-naphthoquinone)

Name: Naphthazarin
Brand: MedChemExpress
SKU: HY-N7526
Rating: 4.5 (1 reviews)

Cat. No.: HY-N7526 Purity: 99.59%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Naphthazarin (DHNQ) is a naturally occurring compound. Naphthazarin is effective by various cellular mechanisms including oxidative stress, activation of mitochondrial apoptosis-inducing factor (AIF), depolymerization of microtubules, interference with lysosomal function and p53-dependent p21 activation. Naphthazarin triggers apoptosis and has anti-tumor effects.

For research use only. We do not sell to patients.

Naphthazarin Chemical Structure

CAS No. : 475-38-7

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 82	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 82	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 75	In-stock	Estimated Time of Arrival: December 31
250 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
500 mg		Get quote
1 g		Get quote

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Naphthazarin

•Int J Biol Macromol. 2021 Apr 24.

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References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	0.08 μM Compound: 15	Antiproliferative activity against human A2780 cells after 2 days by alamar-blue assay Antiproliferative activity against human A2780 cells after 2 days by alamar-blue assay	[PMID: 19028102]
BT-549	GI₅₀	0.43 μM Compound: 1	Antiproliferative activity against human BT-549 cells measured after 72 hrs by SRB assay Antiproliferative activity against human BT-549 cells measured after 72 hrs by SRB assay	[PMID: 35687347]
CAL-51	GI₅₀	0.56 μM Compound: 1	Antiproliferative activity against human CAL-51 cells measured after 72 hrs by SRB assay Antiproliferative activity against human CAL-51 cells measured after 72 hrs by SRB assay	[PMID: 35687347]
HCC1954	GI₅₀	0.49 μM Compound: 1	Antiproliferative activity against human HCC1954 cells measured after 72 hrs by SRB assay Antiproliferative activity against human HCC1954 cells measured after 72 hrs by SRB assay	[PMID: 35687347]
HCT-116	GI₅₀	0.16 μM Compound: 1	Antiproliferative activity against human HCT-116 cells measured after 72 hrs by SRB assay Antiproliferative activity against human HCT-116 cells measured after 72 hrs by SRB assay	[PMID: 35687347]
HL-60	IC₅₀	0.8 μM Compound: 2	Cytotoxicity in human HL60 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity in human HL60 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 28987605]
HL-60	IC₅₀	0.93 μM Compound: 2	Cytotoxicity in human HL60 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity in human HL60 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 28987605]
K562	GI₅₀	1.7 μM Compound: 1	Antiproliferative activity against human K562 cells measured after 72 hrs by SRB assay Antiproliferative activity against human K562 cells measured after 72 hrs by SRB assay	[PMID: 35687347]
MCF7	GI₅₀	0.43 μM Compound: 1	Antiproliferative activity against human MCF7 cells measured after 72 hrs by SRB assay Antiproliferative activity against human MCF7 cells measured after 72 hrs by SRB assay	[PMID: 35687347]
MOLM-13	GI₅₀	0.28 μM Compound: 1	Antiproliferative activity against human MOLM-13 cells measured after 72 hrs by SRB assay Antiproliferative activity against human MOLM-13 cells measured after 72 hrs by SRB assay	[PMID: 35687347]
MV4-11	GI₅₀	0.19 μM Compound: 1	Antiproliferative activity against human MV4-11 cells measured after 72 hrs by SRB assay Antiproliferative activity against human MV4-11 cells measured after 72 hrs by SRB assay	[PMID: 35687347]

Molecular Weight

190.15

Formula

C₁₀H₆O₄

CAS No.

475-38-7

Appearance

Solid

Color

Brown to black

SMILES

O=C1C=CC(C2=C1C(O)=CC=C2O)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 7.14 mg/mL (37.55 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.2590 mL	26.2950 mL	52.5901 mL
5 mM	1.0518 mL	5.2590 mL	10.5180 mL
10 mM	0.5259 mL	2.6295 mL	5.2590 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 0.71 mg/mL (3.73 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.71 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.1 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.59%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (255 KB)

Handling Instructions (2659 KB)

References

[1]. Seon Young Choi, et al. Naphthazarin has a protective effect on the 1-methyl-4-phenyl-1,2,3,4-tetrahydropyridine-induced Parkinson's disease model. J Neurosci Res. 2012 Sep;90(9):1842-9. [Content Brief]

[2]. Omar Aljanadi, et al. Stimulation of Suicidal Erythrocyte Death by Naphthazarin. Basic Clin Pharmacol Toxicol. 2015 Dec;117(6):369-74. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.2590 mL	26.2950 mL	52.5901 mL	131.4752 mL
	5 mM	1.0518 mL	5.2590 mL	10.5180 mL	26.2950 mL
	10 mM	0.5259 mL	2.6295 mL	5.2590 mL	13.1475 mL
	15 mM	0.3506 mL	1.7530 mL	3.5060 mL	8.7650 mL
	20 mM	0.2630 mL	1.3148 mL	2.6295 mL	6.5738 mL
	25 mM	0.2104 mL	1.0518 mL	2.1036 mL	5.2590 mL
	30 mM	0.1753 mL	0.8765 mL	1.7530 mL	4.3825 mL

Naphthazarin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Naphthazarin475-38-7DHNQ 5,8-Dihydroxy-1,4-naphthoquinoneApoptosisNaturallyoxidativestressmitochondrialAIFp53-dependentp21anti-tumormicrotubuleslysosomalInhibitorinhibitorinhibit

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Naphthazarin (Synonyms: DHNQ; 5,8-Dihydroxy-1,4-naphthoquinone)

Customer Review

Naphthazarin Related Antibodies

1 Publications Citing Use of MCE Naphthazarin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Naphthazarin Related Classifications

Keywords:

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SAFETY DATA SHEET (SDS)

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