NCC-149

Name: NCC-149
Brand: MedChemExpress
SKU: HY-117348
Rating: 4.5 (1 reviews)

Cat. No.: HY-117348 Purity: 99.02%: FAQs
Data Sheet SDS COA Handling Instructions

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NCC-149 is a selective HDAC8 inhibitor and can be used for neural differentiation research.

For research use only. We do not sell to patients.

NCC-149 Chemical Structure

CAS No. : 1316652-41-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 341	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 341	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 310	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 960	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1450	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2200	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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View All HDAC Isoform Specific Products:

View All Isoforms

HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

Biological Activity
Purity & Documentation
References
Customer Review

Description

NCC-149 is a selective HDAC8 inhibitor and can be used for neural differentiation research^[1].

IC₅₀ & Target

HDAC8

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	54 μM Compound: C149	Inhibition of HDAC in human HeLa cells nuclear extracts by fluorometric assay Inhibition of HDAC in human HeLa cells nuclear extracts by fluorometric assay	[PMID: 23116147]
HH	GI₅₀	23 μM Compound: C149	Growth inhibition of human HH cells incubated for 72 hrs by MTS assay Growth inhibition of human HH cells incubated for 72 hrs by MTS assay	[PMID: 23116147]
Jurkat	GI₅₀	7.6 μM Compound: C149	Growth inhibition of human Jurkat cells incubated for 72 hrs by MTS assay Growth inhibition of human Jurkat cells incubated for 72 hrs by MTS assay	[PMID: 23116147]
MT2	GI₅₀	11 μM Compound: C149	Growth inhibition of human MT2 cells incubated for 72 hrs by MTS assay Growth inhibition of human MT2 cells incubated for 72 hrs by MTS assay	[PMID: 23116147]
MT4	GI₅₀	15 μM Compound: C149	Growth inhibition of human MT4 cells incubated for 72 hrs by MTS assay Growth inhibition of human MT4 cells incubated for 72 hrs by MTS assay	[PMID: 23116147]
NB1	GI₅₀	12 μM Compound: C149	Growth inhibition of human NB-1 cells incubated for 72 hrs by MTS assay Growth inhibition of human NB-1 cells incubated for 72 hrs by MTS assay	[PMID: 23116147]
PBMC	GI₅₀	> 100 μM Compound: C149	Growth inhibition of human PBMC PB-N cells incubated for 72 hrs by MTS assay Growth inhibition of human PBMC PB-N cells incubated for 72 hrs by MTS assay	[PMID: 23116147]
PBMC	GI₅₀	> 100 μM Compound: C149	Growth inhibition of human PBMC PB-O cells incubated for 72 hrs by MTS assay Growth inhibition of human PBMC PB-O cells incubated for 72 hrs by MTS assay	[PMID: 23116147]

In Vitro

NCC-149 (5 μM; 3 days) remarkably reduces NeuN expression levels in P19 cells^[1].
NCC-149 (0-40 μM; 4 days) reduces embryoid body size in a dose-dependent manner^[1].
NCC-149 (5 and 20 μM; 24 h) downregulates P19 cell proliferation^[1].
NCC-149 (2.5 and 5 μM; 24 h) leads to cell growth retardation by G2/M phase arrest^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	P19 cells
Concentration:	5 μM
Incubation Time:	3 days
Result:	Remarkably reduced NeuN expression levels.

Cell Proliferation Assay^[1]

Cell Line:	P19 cells
Concentration:	5 and 20 μM
Incubation Time:	24 h
Result:	Downregulated cell proliferation.

Cell Cycle Analysis^[1]

Cell Line:	P19 cells
Concentration:	2.5 and 5 μM
Incubation Time:	24 h
Result:	Led to a significant increase in G2/M phase cells, with a slight decrease in S phase cells.

RT-PCR^[1]

Cell Line:	P19 cells
Concentration:	25 μM or 2.5 and 5 μM
Incubation Time:	4 days (25 μM) or 48 h (2.5 and 5 μM)
Result:	Did not reduce the HDAC8 expression at the mRNA level. Significantly and partially reduced cyclin B1 and cyclin A2 gene expression, respectively.

Molecular Weight

326.37

Formula

C₁₆H₁₄N₄O₂S

CAS No.

1316652-41-1

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(NO)C1=CC=CC(C2=CN(CSC3=CC=CC=C3)N=N2)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (383.00 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0640 mL	15.3200 mL	30.6401 mL
5 mM	0.6128 mL	3.0640 mL	6.1280 mL
10 mM	0.3064 mL	1.5320 mL	3.0640 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.14%

Select Batch:

Data Sheet (272 KB) SDS (251 KB)

COA (245 KB) RP-HPLC (244 KB) LCMS (112 KB)

Handling Instructions (2659 KB)

References

[1]. Katayama S, et al. HDAC8 regulates neural differentiation through embryoid body formation in P19 cells. Biochem Biophys Res Commun. 2018 Mar 25;498(1):45-51. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0640 mL	15.3200 mL	30.6401 mL	76.6002 mL
	5 mM	0.6128 mL	3.0640 mL	6.1280 mL	15.3200 mL
	10 mM	0.3064 mL	1.5320 mL	3.0640 mL	7.6600 mL
	15 mM	0.2043 mL	1.0213 mL	2.0427 mL	5.1067 mL
	20 mM	0.1532 mL	0.7660 mL	1.5320 mL	3.8300 mL
	25 mM	0.1226 mL	0.6128 mL	1.2256 mL	3.0640 mL
	30 mM	0.1021 mL	0.5107 mL	1.0213 mL	2.5533 mL
	40 mM	0.0766 mL	0.3830 mL	0.7660 mL	1.9150 mL
	50 mM	0.0613 mL	0.3064 mL	0.6128 mL	1.5320 mL
	60 mM	0.0511 mL	0.2553 mL	0.5107 mL	1.2767 mL
	80 mM	0.0383 mL	0.1915 mL	0.3830 mL	0.9575 mL
	100 mM	0.0306 mL	0.1532 mL	0.3064 mL	0.7660 mL

NCC-149 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NCC-1491316652-41-1NCC149NCC 149HDACHistone deacetylasesembryoid bodyNeuNP19neural differentiationInhibitorinhibitorinhibit

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NCC-149

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NCC-149 Related Antibodies

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NCC-149 Related Antibodies

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Complete Stock Solution Preparation Table

NCC-149 Related Classifications

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