NCI126224

Cat. No.: HY-156060 Purity: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

NCI126224 is a TLR4 signaling inhibitor. NCI126224 suppress LPS (HY-D1056)-induced production of NF-κB, TNF-α, IL-1β, and NO in the nanomolar-low micromolar range. NCI126224 can be used for the research of inflammatory diseases.

For research use only. We do not sell to patients.

NCI126224 Chemical Structure

CAS No. : 65974-52-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 104	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 104	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 155	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 295	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 760	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	0.31 μM Compound: 1, NCI126224	Inhibition of TLR4 in LPS-stimulated mouse RAW264.7 cells assessed as reduction of NO production after 24 hrs Inhibition of TLR4 in LPS-stimulated mouse RAW264.7 cells assessed as reduction of NO production after 24 hrs	[PMID: 22985959]
RAW264.7	IC₅₀	0.42 μM Compound: 1, NCI126224	Inhibition of TLR4 in LPS-stimulated mouse RAW264.7 cells assessed as reduction of IL1-beta production after 24 hrs by ELISA Inhibition of TLR4 in LPS-stimulated mouse RAW264.7 cells assessed as reduction of IL1-beta production after 24 hrs by ELISA	[PMID: 22985959]
RAW264.7	IC₅₀	1.54 μM Compound: 1, NCI126224	Inhibition of TLR4 in LPS-stimulated mouse RAW264.7 cells assessed as reduction of TNFalpha production after 24 hrs by ELISA Inhibition of TLR4 in LPS-stimulated mouse RAW264.7 cells assessed as reduction of TNFalpha production after 24 hrs by ELISA	[PMID: 22985959]

Molecular Weight

265.22

Formula

C₁₂H₁₁NO₆

CAS No.

65974-52-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(/C(C(OC)=O)=C\C1=C([N+]([O-])=O)C=CC=C1)OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (471.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7705 mL	18.8523 mL	37.7045 mL
5 mM	0.7541 mL	3.7705 mL	7.5409 mL
10 mM	0.3770 mL	1.8852 mL	3.7705 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (254 KB) HNMR (257 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang S, et, al. Selection, synthesis, and anti-inflammatory evaluation of the arylidene malonate derivatives as TLR4 signaling inhibitors. Bioorg Med Chem. 2012 Oct 15;20(20):6073-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7705 mL	18.8523 mL	37.7045 mL	94.2614 mL
	5 mM	0.7541 mL	3.7705 mL	7.5409 mL	18.8523 mL
	10 mM	0.3770 mL	1.8852 mL	3.7705 mL	9.4261 mL
	15 mM	0.2514 mL	1.2568 mL	2.5136 mL	6.2841 mL
	20 mM	0.1885 mL	0.9426 mL	1.8852 mL	4.7131 mL
	25 mM	0.1508 mL	0.7541 mL	1.5082 mL	3.7705 mL
	30 mM	0.1257 mL	0.6284 mL	1.2568 mL	3.1420 mL
	40 mM	0.0943 mL	0.4713 mL	0.9426 mL	2.3565 mL
	50 mM	0.0754 mL	0.3770 mL	0.7541 mL	1.8852 mL
	60 mM	0.0628 mL	0.3142 mL	0.6284 mL	1.5710 mL
	80 mM	0.0471 mL	0.2357 mL	0.4713 mL	1.1783 mL
	100 mM	0.0377 mL	0.1885 mL	0.3770 mL	0.9426 mL

NCI126224 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NCI12622465974-52-9NCI 126224NCI-126224Toll-like Receptor (TLR)LPSNF-κBTNF-αIL-1βNOinflammatory diseasesInhibitorinhibitorinhibit

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