2. Metabolic Enzyme/Protease Anti-infection Cell Cycle/DNA Damage
  3. Endogenous Metabolite Enterovirus HCV Topoisomerase SARS-CoV
  4. NHC-diphosphate triammonium

NHC-diphosphate triammonium is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a diphosphate form. NHC-diphosphate triammonium is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA.

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NHC-diphosphate triammonium Chemical Structure

NHC-diphosphate triammonium Chemical Structure

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5 mg USD 800 In-stock
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Other Forms of NHC-diphosphate triammonium:

NHC-diphosphate triammonium Related Antibodies

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1 Publications Citing Use of MCE NHC-diphosphate triammonium

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

NHC-diphosphate triammonium is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a diphosphate form[1]. NHC-diphosphate triammonium is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA[1][2].

In Vitro

In an intracellular metabolism assay, HCV replicon cells are treated with 10 μM 3H-labeled NHC, and intracellular nucleotide levels are determined after 1, 2 and 8 hours incubations. NHC is rapidly convered into the mono-, di-, and triphosphate forms, and NHC-TP reaches up to 71.12 pM after 8 hours[1].
NHC-triphosphate triammonium (NHC-TP) (5-40 μM) absence leads to full-length polymerization products, it can be a weak alternative substrate. In addition, incorporation of NHC-TP instead of CTP increases the molecular weight of the polymerization product by 16 (one extra oxygen) for each event and an obvious electrophoretic shift is observed in cell-free HCV NS5B polymerization reactions[1].
Huh-7 cells are incubated with (10-50 μM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC. Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP) can be observed, while NHC-triphosphate triammonium (HY-135867) remains the most abundant metabolite[2].
NHC-triphosphate triammonium (NHC-TP) metabolite may directly target the viral polymerase and behave as a nonobligate chain terminator. It plays a prominent role in inhibiting early negative-strand RNA synthesis, either through chain termination or mutagenesis, which may in turn interfere with correct replicase complex formation.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NHC-diphosphate triammonium Related Antibodies
Molecular Weight

470.27

Formula

C9H24N6O12P2
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]([C@@H]1O)[C@@](N(C=C/C2=N\O)C(N2)=O)([H])O[C@@H]1CO[P](O[P](O)(O)=O)(O)=O.N.N.N
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 160 mg/mL (340.23 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1264 mL 10.6322 mL 21.2644 mL
5 mM 0.4253 mL 2.1264 mL 4.2529 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (106.32 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.1264 mL 10.6322 mL 21.2644 mL 53.1610 mL
5 mM 0.4253 mL 2.1264 mL 4.2529 mL 10.6322 mL
10 mM 0.2126 mL 1.0632 mL 2.1264 mL 5.3161 mL
15 mM 0.1418 mL 0.7088 mL 1.4176 mL 3.5441 mL
20 mM 0.1063 mL 0.5316 mL 1.0632 mL 2.6580 mL
25 mM 0.0851 mL 0.4253 mL 0.8506 mL 2.1264 mL
30 mM 0.0709 mL 0.3544 mL 0.7088 mL 1.7720 mL
40 mM 0.0532 mL 0.2658 mL 0.5316 mL 1.3290 mL
50 mM 0.0425 mL 0.2126 mL 0.4253 mL 1.0632 mL
60 mM 0.0354 mL 0.1772 mL 0.3544 mL 0.8860 mL
80 mM 0.0266 mL 0.1329 mL 0.2658 mL 0.6645 mL
100 mM 0.0213 mL 0.1063 mL 0.2126 mL 0.5316 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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NHC-diphosphate triammonium Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NHC-diphosphate triammoniumEndogenous MetaboliteEnterovirusHCVTopoisomeraseSARS-CoVRhinovirusHRVHRVsHEVHEVsHepatitis C virusSARS coronavirusEnterovirus,VEEVCHIKVvenezuelanequineencephalitisChikungunyaintracellularmetaboliteInhibitorinhibitorinhibit

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