2. Metabolic Enzyme/Protease Anti-infection Cell Cycle/DNA Damage
  3. Endogenous Metabolite Enterovirus HCV Topoisomerase SARS-CoV
  4. NHC-diphosphate

NHC-diphosphate is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a diphosphate form. NHC is a pyrimidine ribonucleoside and behaves as a potent anti-virus agent. NHC effectively inhibits the replication of venezuelan equine encephalitis virus (VEEV), Chikungunya virus (CHIKV)  and hepatitis C virus (HCV).

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NHC-diphosphate Chemical Structure

NHC-diphosphate Chemical Structure

CAS No. : 39023-73-9

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1 mg USD 380 In-stock
5 mg USD 800 In-stock
10 mg USD 1200 In-stock
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Other Forms of NHC-diphosphate:

NHC-diphosphate Related Antibodies

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Description

NHC-diphosphate is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a diphosphate form[1]. NHC is a pyrimidine ribonucleoside and behaves as a potent anti-virus agent. NHC effectively inhibits the replication of venezuelan equine encephalitis virus (VEEV), Chikungunya virus (CHIKV)  and hepatitis C virus (HCV)[1].

In Vitro

Huh-7 cells are incubated with (10-50 μM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC.
Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP; HY-135867D) can be observed, while NHC-triphosphate remains the most abundant metabolite.[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NHC-diphosphate Related Antibodies
Molecular Weight

419.18

Formula

C9H15N3O12P2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]([C@@H]1O)[C@@](N(C=C/C2=N\O)C(N2)=O)([H])O[C@@H]1CO[P](O[P](O)(O)=O)(O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 160 mg/mL (381.70 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3856 mL 11.9281 mL 23.8561 mL
5 mM 0.4771 mL 2.3856 mL 4.7712 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.3856 mL 11.9281 mL 23.8561 mL 59.6403 mL
5 mM 0.4771 mL 2.3856 mL 4.7712 mL 11.9281 mL
10 mM 0.2386 mL 1.1928 mL 2.3856 mL 5.9640 mL
15 mM 0.1590 mL 0.7952 mL 1.5904 mL 3.9760 mL
20 mM 0.1193 mL 0.5964 mL 1.1928 mL 2.9820 mL
25 mM 0.0954 mL 0.4771 mL 0.9542 mL 2.3856 mL
30 mM 0.0795 mL 0.3976 mL 0.7952 mL 1.9880 mL
40 mM 0.0596 mL 0.2982 mL 0.5964 mL 1.4910 mL
50 mM 0.0477 mL 0.2386 mL 0.4771 mL 1.1928 mL
60 mM 0.0398 mL 0.1988 mL 0.3976 mL 0.9940 mL
80 mM 0.0298 mL 0.1491 mL 0.2982 mL 0.7455 mL
100 mM 0.0239 mL 0.1193 mL 0.2386 mL 0.5964 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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NHC-diphosphate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NHC-diphosphate39023-73-9Endogenous MetaboliteEnterovirusHCVTopoisomeraseSARS-CoVRhinovirusHRVHRVsHEVHEVsHepatitis C virusSARS coronavirusintracellularmetaboliteVEEVCHIKVInhibitorinhibitorinhibit

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