EIDD-1931 (Synonyms: β-D-N4-hydroxycytidine; NHC)

Name: EIDD-1931
Brand: MedChemExpress
SKU: HY-125033
Rating: 5.0 (21 reviews)

Cat. No.: HY-125033 Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

EIDD-1931 (Beta-d-N4-hydroxycytidine; NHC) is a novel nucleoside analog and behaves as a potent anti-virus agent. EIDD-1931 effectively inhibits the replication activity of venezuelan equine encephalitis virus (VEEV), Chikungunya virus (CHIKV) and hepatitis C virus (HCV).

For research use only. We do not sell to patients.

EIDD-1931 Chemical Structure

CAS No. : 3258-02-4

Size	Price	Stock	Quantity
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5 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 21 publication(s) in Google Scholar

Other Forms of EIDD-1931:

EIDD-1931-d₂ Get quote

21 Publications Citing Use of MCE EIDD-1931

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
BHK-21	CC₅₀	5.75 μM Compound: 1; NHC	Cytotoxicity against hamster BHK-21 cells incubated for 4 days by MTS assay Cytotoxicity against hamster BHK-21 cells incubated for 4 days by MTS assay	[PMID: 36764470]
MDCK	CC₅₀	> 100 μM Compound: 1; NHC	Cytotoxicity against MDCK cells assessed as reduction in cell viability measured for 48 hrs by MTT assay Cytotoxicity against MDCK cells assessed as reduction in cell viability measured for 48 hrs by MTT assay	[PMID: 36764470]
Vero	CC₅₀	> 100 μM Compound: NHC	Cytotoxicity in African green monkey Vero cells assessed as reduction in cell viability measured after 5 days by resazurin dye based assay Cytotoxicity in African green monkey Vero cells assessed as reduction in cell viability measured after 5 days by resazurin dye based assay	[PMID: 33894564]
Vero	CC₅₀	16.5 μM Compound: 1; NHC	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 36764470]
Vero C1008	CC₅₀	> 10 μM Compound: 25; NHC, EIDD-1931	Cytotoxicity against African green monkey Vero E6 cells infected with SARS CoV-2 clinical isolate 2019-nCoV/USA-WA1/2020 incubated for 48 hrs by CellTiter-Glo assay Cytotoxicity against African green monkey Vero E6 cells infected with SARS CoV-2 clinical isolate 2019-nCoV/USA-WA1/2020 incubated for 48 hrs by CellTiter-Glo assay	[PMID: 33186044]

In Vitro

Beta-d-N4-hydroxycytidine is an anti-VEEV (venezuelan equine encephalitis virus) agent with EC₅₀, EC₉₀, and EC₉₉ are 0.426, 1.036, and 2.5 μM, respectively^[1].
Beta-d-N4-hydroxycytidine inhibits CHIKV replicon activity and the 50% effective concentration (EC₅₀) s 0.8 μM in the Huh-7–CHIKV replicon cell line. Similar results is presented with the replicon in BHK-21 cells (EC₅₀=1.8 μM)^[2].
NHC has no cytotoxicity for NHC in the Huh-7 cell culture system until up to 100 μM using MTT assays. The 50% cytotoxic concentration (CCsub>50) values for NHC are determined to be 30.6 μM, 7.7 μM, and 2.5 μM in peripheral blood mononuclear (PBM), Vero, and CEM cells, respectively^[2].
NHC behaves as a pyrimidine analog, NHC-mediated inhibition of the CHIKV replicon can be abrogated by the addition of exogenous nucleosides, such as pyrimidines C and U, but dA, dC, dG, dU, or T has no impact on the replicon. Pyrimidines A and G contributes to replicon inhibition both in the presence and in the absence of NHC^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

259.22

Formula

C₉H₁₃N₃O₆

CAS No.

3258-02-4

Appearance

Solid

Color

White to off-white

SMILES

O=C1N/C(C=CN1[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)=N/O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (385.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 25 mg/mL (96.44 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8577 mL	19.2886 mL	38.5773 mL
5 mM	0.7715 mL	3.8577 mL	7.7155 mL
10 mM	0.3858 mL	1.9289 mL	3.8577 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (8.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (8.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (277 KB) SDS (604 KB)

COA (249 KB) RP-HPLC (219 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Urakova N, et al. β-d-N⁴-Hydroxycytidine Is a Potent Anti-alphavirus Compound That Induces a High Level of Mutations in the Viral Genome. J Virol. 2018 Jan 17;92(3). pii: e01965-17. [Content Brief]

[2]. Brenda I Hernandez-Santiago, et al. Metabolism of the anti-hepatitis C virus nucleoside beta-D-N4-hydroxycytidine in different liver cells. Antimicrob Agents Chemother. 2004 Dec;48(12):4636-42 [Content Brief]

[3]. Maryam Ehteshami, et al. Characterization of β-d- N4-Hydroxycytidine as a Novel Inhibitor of Chikungunya Virus. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.8577 mL	19.2886 mL	38.5773 mL	96.4432 mL
	5 mM	0.7715 mL	3.8577 mL	7.7155 mL	19.2886 mL
	10 mM	0.3858 mL	1.9289 mL	3.8577 mL	9.6443 mL
	15 mM	0.2572 mL	1.2859 mL	2.5718 mL	6.4295 mL
	20 mM	0.1929 mL	0.9644 mL	1.9289 mL	4.8222 mL
	25 mM	0.1543 mL	0.7715 mL	1.5431 mL	3.8577 mL
	30 mM	0.1286 mL	0.6430 mL	1.2859 mL	3.2148 mL
	40 mM	0.0964 mL	0.4822 mL	0.9644 mL	2.4111 mL
	50 mM	0.0772 mL	0.3858 mL	0.7715 mL	1.9289 mL
	60 mM	0.0643 mL	0.3215 mL	0.6430 mL	1.6074 mL
	80 mM	0.0482 mL	0.2411 mL	0.4822 mL	1.2055 mL
DMSO	100 mM	0.0386 mL	0.1929 mL	0.3858 mL	0.9644 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

EIDD-1931 Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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EIDD-1931 (Synonyms: β-D-N4-hydroxycytidine; NHC)

Customer Review

Other Forms of EIDD-1931:

EIDD-1931 Related Antibodies

21 Publications Citing Use of MCE EIDD-1931

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Topoisomerase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

EIDD-1931 Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

EIDD-1931 Related Classifications

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