NICE-01 (Synonyms: AP1867-PEG2-JQ1; AP-PEG2-JQ1)

Cat. No.: HY-156214 Purity: 99.53%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

NICE-01 (AP1867-PEG2-JQ1; AP-PEG2-JQ1) is a bifunctional compound that bind to proteins in separate cellular compartments that can induce nuclear import of cytosolic cargoes, using nuclear-localized bromodomain-containing protein 4 (BRD4) as a “carrier” for co-import and nuclear trapping of cytosolic proteins.

For research use only. We do not sell to patients.

NICE-01 Chemical Structure

CAS No. : 2982819-94-1

Size	Price	Stock	Quantity
1 mg	USD 540	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 1200	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1940	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Description

Molecular Weight

1206.83

Formula

C₆₃H₇₆ClN₇O₁₃S

CAS No.

2982819-94-1

Appearance

Solid

Color

Light yellow to yellow

SMILES

CC(C(C(C(C=C1)=CC=C1Cl)=N[C@H]2CC(NCCOCCOCCNC(COC3=CC([C@@H](CCC(C=C4)=CC(OC)=C4OC)OC([C@H](CCCC5)N5C([C@H](C(C=C6OC)=CC(OC)=C6OC)CC)=O)=O)=CC=C3)=O)=O)=C(S7)N8C2=NN=C8C)=C7C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (82.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.8286 mL	4.1431 mL	8.2862 mL
5 mM	0.1657 mL	0.8286 mL	1.6572 mL
10 mM	0.0829 mL	0.4143 mL	0.8286 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.53%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (249 KB) HNMR (449 KB) RP-HPLC (258 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. William J. Gibson, et al. Bifunctional small molecules that induce nuclear localization and targeted transcriptional regulation. Version 1. bioRxiv. Preprint. 2023 Jul 7.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.8286 mL	4.1431 mL	8.2862 mL	20.7154 mL
	5 mM	0.1657 mL	0.8286 mL	1.6572 mL	4.1431 mL
	10 mM	0.0829 mL	0.4143 mL	0.8286 mL	2.0715 mL
	15 mM	0.0552 mL	0.2762 mL	0.5524 mL	1.3810 mL
	20 mM	0.0414 mL	0.2072 mL	0.4143 mL	1.0358 mL
	25 mM	0.0331 mL	0.1657 mL	0.3314 mL	0.8286 mL
	30 mM	0.0276 mL	0.1381 mL	0.2762 mL	0.6905 mL
	40 mM	0.0207 mL	0.1036 mL	0.2072 mL	0.5179 mL
	50 mM	0.0166 mL	0.0829 mL	0.1657 mL	0.4143 mL
	60 mM	0.0138 mL	0.0691 mL	0.1381 mL	0.3453 mL
	80 mM	0.0104 mL	0.0518 mL	0.1036 mL	0.2589 mL

NICE-01 Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NICE-012982819-94-1AP1867-PEG2-JQ1 AP-PEG2-JQ1NICE01NICE 01OthersBRD4Cytosolic ProteinNucleusInhibitorinhibitorinhibit

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