2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. Calcium Channel
  4. NNC 55-0396

NNC 55-0396  (Synonyms: NNC 55-0396 dihydrochloride)

Cat. No.: HY-50722 Purity: ≥99.0%
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NNC 55-0396 is a highly selective T-type calcium channel blocker with an IC50 value of 6.8 μM for Cav3.1 T-type channels. NNC 55-0396 can be used for the research of neurological disease research.

For research use only. We do not sell to patients.

NNC 55-0396 Chemical Structure

NNC 55-0396 Chemical Structure

CAS No. : 357400-13-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 199 In-stock
Solution
10 mM * 1 mL in DMSO USD 199 In-stock
Solid
1 mg USD 76 In-stock
5 mg USD 160 In-stock
10 mg USD 260 In-stock
50 mg USD 700 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 4 publication(s) in Google Scholar

Other Forms of NNC 55-0396:

NNC 55-0396 Related Antibodies

Top Publications Citing Use of Products

View All Calcium Channel Isoform Specific Products:

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N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

NNC 55-0396 is a highly selective T-type calcium channel blocker with an IC50 value of 6.8 μM for Cav3.1 T-type channels. NNC 55-0396 can be used for the research of neurological disease research[1][2].

IC50 & Target

T-type calcium channel

 

In Vitro

NNC 55-0396 (0.1-1000 μM) shows no inhibition to the HVA Ca2+ currents in INS-1 cells[1].
NNC 55-0396 (1-100 μM) blocks more than 50% of the T-type Ca2+ current at 8 μM in HEK 293/α1G cells[1].
NNC 55-0396 blocks T-type Ca2+ current with an IC50 value of 6.8 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NNC 55-0396 Related Antibodies
In Vivo

NNC 55-0396 (20 mg/kg; i.p. once) suppresses tremor in GABAA subunit α1-null mice and harmaline mouse models[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: GABAA receptor α1 subunit null mouse model[2]
Dosage: 20 mg/kg
Administration: Intraperitoneal injection; 20 mg/kg, once
Result: Suppressed tremor in GABAA subunit α1-null mice.
Molecular Weight

564.56

Formula

C30H40Cl2FN3O2
CAS No.
Appearance

Solid

Color

Off-white to yellow

SMILES

O=C(C1CC1)O[C@@]2(CCN(CCCC3=NC4=CC=CC=C4N3)C)[C@@H](C(C)C)C5=C(C=C(F)C=C5)CC2.[H]Cl.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (177.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7713 mL 8.8565 mL 17.7129 mL
5 mM 0.3543 mL 1.7713 mL 3.5426 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (3.68 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (3.68 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥99.0%

COA (249 KB) HNMR (130 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7713 mL 8.8565 mL 17.7129 mL 44.2823 mL
5 mM 0.3543 mL 1.7713 mL 3.5426 mL 8.8565 mL
10 mM 0.1771 mL 0.8856 mL 1.7713 mL 4.4282 mL
15 mM 0.1181 mL 0.5904 mL 1.1809 mL 2.9522 mL
20 mM 0.0886 mL 0.4428 mL 0.8856 mL 2.2141 mL
25 mM 0.0709 mL 0.3543 mL 0.7085 mL 1.7713 mL
30 mM 0.0590 mL 0.2952 mL 0.5904 mL 1.4761 mL
40 mM 0.0443 mL 0.2214 mL 0.4428 mL 1.1071 mL
50 mM 0.0354 mL 0.1771 mL 0.3543 mL 0.8856 mL
60 mM 0.0295 mL 0.1476 mL 0.2952 mL 0.7380 mL
80 mM 0.0221 mL 0.1107 mL 0.2214 mL 0.5535 mL
100 mM 0.0177 mL 0.0886 mL 0.1771 mL 0.4428 mL
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NNC 55-0396 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NNC 55-0396357400-13-6Calcium ChannelCa2+ channelsCa channelsHEK 293harmalinemiceINS-1Inhibitorinhibitorinhibit

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