Physalin F

Name: Physalin F
Brand: MedChemExpress
SKU: HY-N7696
Rating: 4.5 (1 reviews)

Cat. No.: HY-N7696 Purity: 98.17%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Physalin F is a secosteroid with potent anti-inflammatory and immunomodulatory activities. Physalin F induces apoptosis of PBMC, decreasing the spontaneous proliferation and cytokine production caused by Human T-lymphotropic virus type 1 (HTLV-1) infection.

For research use only. We do not sell to patients.

Physalin F Chemical Structure

CAS No. : 57423-71-9

Size	Price	Stock	Quantity
1 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 50 μM Compound: 22	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay	[PMID: 35608269]
HepG2	IC₅₀	> 50 μM Compound: 22	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay	[PMID: 35608269]
HT-1080	IC₅₀	2.41 μM Compound: Physalin F	Antiproliferative activity against human HT-1080 cells assessed as reduction in cell proliferation measured after 48 hrs by CCK8 assay Antiproliferative activity against human HT-1080 cells assessed as reduction in cell proliferation measured after 48 hrs by CCK8 assay	[PMID: 33735687]
L02	IC₅₀	> 50 μM Compound: 22	Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay	[PMID: 35608269]
MCF7	IC₅₀	> 50 μM Compound: 22	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay	[PMID: 35608269]
MCF7	IC₅₀	0.6 μM Compound: 8	Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay	[PMID: 33586438]
NCI-H460	IC₅₀	1 μM Compound: 8	Cytotoxicity against human NCI-H460 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay Cytotoxicity against human NCI-H460 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay	[PMID: 33586438]
PC-3	IC₅₀	1 μM Compound: 8	Cytotoxicity against human PC-3 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay Cytotoxicity against human PC-3 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay	[PMID: 33586438]
RAW264.7	IC₅₀	> 50 μM Compound: 22	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay	[PMID: 35608269]
RAW264.7	IC₅₀	7.9 μM Compound: 22	Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-stimulated NO production pretreated with LPS followed by compund addition by Griess reagent based microplate reader assay Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-stimulated NO production pretreated with LPS followed by compund addition by Griess reagent based microplate reader assay	[PMID: 35608269]
SF-268	IC₅₀	0.5 μM Compound: 8	Cytotoxicity against human SF-268 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay Cytotoxicity against human SF-268 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay	[PMID: 33586438]
SGC-7901	IC₅₀	> 50 μM Compound: 22	Antiproliferative activity against human SGC-7901 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay Antiproliferative activity against human SGC-7901 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay	[PMID: 35608269]
WI-38	IC₅₀	3.5 μM Compound: 8	Cytotoxicity against human WI-38 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay Cytotoxicity against human WI-38 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay	[PMID: 33586438]

In Vitro

Physalin F is a seco-steroid from Physalis angulate L.^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

526.53

Formula

C₂₈H₃₀O₁₀

CAS No.

57423-71-9

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]([C@@](OC1=O)([H])C2)(OC3=O)[C@@]([C@@]3(CC[C@@]4([H])[C@@]5([H])C[C@@H](O6)[C@]6(CC=C7)[C@@]4(C7=O)C)O)(O[C@@]58C9=O)[C@]9([H])[C@]2([C@@]1([H])CO8)C

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (189.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8992 mL	9.4961 mL	18.9923 mL
5 mM	0.3798 mL	1.8992 mL	3.7985 mL
10 mM	0.1899 mL	0.9496 mL	1.8992 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.11 mg/mL (2.11 mM); Clear solution

This protocol yields a clear solution of ≥ 1.11 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.1 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1 mg/mL (1.90 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.17%

Select Batch:

Data Sheet (278 KB) SDS (252 KB)

COA (259 KB) HNMR (174 KB) RP-HPLC (299 KB)

Handling Instructions (2659 KB)

References

[1]. Pinto LA, et al. Physalin F, a seco-steroid from Physalis angulata L., has immunosuppressive activity in peripheral blood mononuclear cells from patients with HTLV1-associated myelopathy. Biomed Pharmacother. 2016;79:129-134. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8992 mL	9.4961 mL	18.9923 mL	47.4807 mL
	5 mM	0.3798 mL	1.8992 mL	3.7985 mL	9.4961 mL
	10 mM	0.1899 mL	0.9496 mL	1.8992 mL	4.7481 mL
	15 mM	0.1266 mL	0.6331 mL	1.2662 mL	3.1654 mL
	20 mM	0.0950 mL	0.4748 mL	0.9496 mL	2.3740 mL
	25 mM	0.0760 mL	0.3798 mL	0.7597 mL	1.8992 mL
	30 mM	0.0633 mL	0.3165 mL	0.6331 mL	1.5827 mL
	40 mM	0.0475 mL	0.2374 mL	0.4748 mL	1.1870 mL
	50 mM	0.0380 mL	0.1899 mL	0.3798 mL	0.9496 mL
	60 mM	0.0317 mL	0.1583 mL	0.3165 mL	0.7913 mL
	80 mM	0.0237 mL	0.1187 mL	0.2374 mL	0.5935 mL
	100 mM	0.0190 mL	0.0950 mL	0.1899 mL	0.4748 mL

Physalin F Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Physalin F57423-71-9ApoptosisinflammatoryimmunomodulatoryPBMCproliferationcytokineHTLV-1infectionInhibitorinhibitorinhibit

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Physalin F

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Complete Stock Solution Preparation Table

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