2. Apoptosis Metabolic Enzyme/Protease
  3. Glutathione Peroxidase
  4. PKUMDL-LC-101-D04

PKUMDL-LC-101-D04  (Synonyms: GPX4-Activator-1d4)

Cat. No.: HY-115627 Purity: 99.43%
COA Handling Instructions

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PKUMDL-LC-101-D04 (GPX4-Activator-1d4) is a glutathione peroxidase 4 (GPX4) allosteric activator (pEC50=4.7). PKUMDL-LC-101-D04 can inhibit ferroptosis and inflammation.

For research use only. We do not sell to patients.

PKUMDL-LC-101-D04 Chemical Structure

PKUMDL-LC-101-D04 Chemical Structure

CAS No. : 2143896-83-5

Size Price Stock Quantity
1 mg USD 100 In-stock
5 mg USD 290 In-stock
10 mg USD 411 In-stock
25 mg USD 700 In-stock
50 mg USD 980 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

PKUMDL-LC-101-D04 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

PKUMDL-LC-101-D04 (GPX4-Activator-1d4) is a glutathione peroxidase 4 (GPX4) allosteric activator (pEC50=4.7). PKUMDL-LC-101-D04 can inhibit ferroptosis and inflammation[1][2].

In Vitro

PKUMDL-LC-101-D04 (20 μM) increases the activity of GPX4 by 150% in cell-free experiments[1].
PKUMDL-LC-101-D04 (61 μM) boosted GPX4 activity to 150% in mouse embryonic fibroblast (MEF) cell extracts[1].
PKUMDL-LC-101-D04 (0-150 μM) activates GPX4 in cells and protects against the toxic effects of cholesterol peroxide[1].
PKUMDL-LC-101-D04 (200 μM,1 h) can inhibit cell death induced by cholesterol peroxide, which is a specific substrate of GPX4[1].
PKUMDL-LC-101-D04 (65 μM, 24 h) does not reduce ROS production in 661 W cells[2].
PKUMDL-LC-101-D04 significantly increased the levels of MDA and iron ions in 661 W cells while reducing mitochondrial shrinkage[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PKUMDL-LC-101-D04 Related Antibodies
Molecular Weight

378.94

Formula

C14H23ClN4O2S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC=C(NC(NC2CCCC2)=S)C=C1)(NCCN)=O.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (659.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6389 mL 13.1947 mL 26.3894 mL
5 mM 0.5278 mL 2.6389 mL 5.2779 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.08 mg/mL (5.49 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.43%

COA (247 KB) HNMR (126 KB) LCMS (99 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6389 mL 13.1947 mL 26.3894 mL 65.9735 mL
5 mM 0.5278 mL 2.6389 mL 5.2779 mL 13.1947 mL
10 mM 0.2639 mL 1.3195 mL 2.6389 mL 6.5974 mL
15 mM 0.1759 mL 0.8796 mL 1.7593 mL 4.3982 mL
20 mM 0.1319 mL 0.6597 mL 1.3195 mL 3.2987 mL
25 mM 0.1056 mL 0.5278 mL 1.0556 mL 2.6389 mL
30 mM 0.0880 mL 0.4398 mL 0.8796 mL 2.1991 mL
40 mM 0.0660 mL 0.3299 mL 0.6597 mL 1.6493 mL
50 mM 0.0528 mL 0.2639 mL 0.5278 mL 1.3195 mL
60 mM 0.0440 mL 0.2199 mL 0.4398 mL 1.0996 mL
80 mM 0.0330 mL 0.1649 mL 0.3299 mL 0.8247 mL
100 mM 0.0264 mL 0.1319 mL 0.2639 mL 0.6597 mL
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PKUMDL-LC-101-D04 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PKUMDL-LC-101-D042143896-83-5GPX4-Activator-1d4Glutathione PeroxidaseGPX4inflammationferroptosis-related diseasesregulatorInhibitorinhibitorinhibit

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