Pladienolide B

Name: Pladienolide B
Brand: MedChemExpress
SKU: HY-16399
Rating: 4.5 (1 reviews)

Cat. No.: HY-16399 Purity: 99.20%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Pladienolide B is a potent cancer cell growth inhibitor that targets the SF3B1 subunit of the spliceosome. Pladienolide B exerts antitumor activities mediated through the inhibition of pre-mRNA splicing. Pladienolide B induces apoptosis.

For research use only. We do not sell to patients.

Pladienolide B Chemical Structure

CAS No. : 445493-23-2

Size	Price	Stock	Quantity
100 μg		In-stock	Estimated Time of Arrival: December 31
500 μg		In-stock	Estimated Time of Arrival: December 31

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Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Pladienolide B

•Res Sq. 2024 Aug 20.

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Pladienolide B (0.1-2 nM; 24-72 hours) inhibits human cervical carcinoma cells viability^[3].
Pladienolide B (0.1-2 nM; 24-48 hours) reduces SF3b1 expression in human cervical carcinoma cells^[3].
Pladienolide B induces (0.1-2 nM; 24 hours) cell cycle arrest and apoptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	HeLa cells
Concentration:	0.1, 0.5, 1, 1.5, 2 nM
Incubation Time:	24, 48, 72 hours
Result:	Significantly decreased cell viability, and the decrease was concentration- and time-dependent.

Apoptosis Analysis^[3]

Cell Line:	HeLa cells
Concentration:	0.1, 0.5, and 2 nM
Incubation Time:	24 hours
Result:	The apoptotic cells were highly induced at 24 hours.

RT-PCR^[3]

Cell Line:	HeLa cells
Concentration:	0.1, 0.5, and 2 nM
Incubation Time:	24, 48 hours
Result:	Induced a time- and concentration-dependent decrease in cellular SF3b1 proteins.

In Vivo

Pladienolide B (2.5-10 mg/kg; i.v.; daily for 5 days) has strong antitumor activities^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female or male BALB/c nu/nu mice (7 weeks of age) (PC-3, OVCAR-3, DU-145, WiDr, and HCT-116, BSY-1 xenografts)^[4]
Dosage:	2.5, 5, and 10 mg/kg
Administration:	I.v.; daily for 5 days
Result:	Showed strong growth inhibitory or regressive activities against these xenografts.

Molecular Weight

536.70

Formula

C₃₀H₄₈O₈

CAS No.

445493-23-2

Appearance

Solid

Color

White to off-white

SMILES

O=C1O[C@H](/C(C)=C/C=C/[C@@H](C)C[C@H]2O[C@@H]2[C@H](C)[C@@H](O)CC)[C@@H](C)/C=C/[C@H](OC(C)=O)[C@](C)(O)CC[C@@H](O)C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (186.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8632 mL	9.3162 mL	18.6324 mL
5 mM	0.3726 mL	1.8632 mL	3.7265 mL
10 mM	0.1863 mL	0.9316 mL	1.8632 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.20%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (247 KB) RP-HPLC (250 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. Effenberger KA, et al. Coherence between cellular responses and in vitro splicing inhibition for the anti-tumor drug pladienolide B and its analogs. J Biol Chem. 2014 Jan 24;289(4):1938-47. [Content Brief]

[2]. Aouida M, et al. CRISPR/Cas9-mediated target validation of the splicing inhibitor Pladienolide B. Biochim Open. 2016 Feb 24;3:72-75. [Content Brief]

[3]. Zhang Q, et al. Inhibition of SF3b1 by pladienolide B evokes cycle arrest, apoptosis induction and p73 splicing in human cervical carcinoma cells. Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1273-1280. [Content Brief]

[4]. Mizui Y, et al. Pladienolides, new substances from culture of Streptomyces platensis Mer-11107. III. In vitro and in vivo antitumor activities. J Antibiot (Tokyo). 2004 Mar;57(3):188-96. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8632 mL	9.3162 mL	18.6324 mL	46.5810 mL
	5 mM	0.3726 mL	1.8632 mL	3.7265 mL	9.3162 mL
	10 mM	0.1863 mL	0.9316 mL	1.8632 mL	4.6581 mL
	15 mM	0.1242 mL	0.6211 mL	1.2422 mL	3.1054 mL
	20 mM	0.0932 mL	0.4658 mL	0.9316 mL	2.3290 mL
	25 mM	0.0745 mL	0.3726 mL	0.7453 mL	1.8632 mL
	30 mM	0.0621 mL	0.3105 mL	0.6211 mL	1.5527 mL
	40 mM	0.0466 mL	0.2329 mL	0.4658 mL	1.1645 mL
	50 mM	0.0373 mL	0.1863 mL	0.3726 mL	0.9316 mL
	60 mM	0.0311 mL	0.1553 mL	0.3105 mL	0.7763 mL
	80 mM	0.0233 mL	0.1165 mL	0.2329 mL	0.5823 mL
	100 mM	0.0186 mL	0.0932 mL	0.1863 mL	0.4658 mL

Pladienolide B Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pladienolide B445493-23-2ApoptosisSF3B1Splicing Factor 3b Subunit 1macrolideantitumorpre-mRNAsplicingcervicalcarcinomaHeLacellsSF3b1cyclearrestInhibitorinhibitorinhibit

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Pladienolide B

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1 Publications Citing Use of MCE Pladienolide B

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Complete Stock Solution Preparation Table

Pladienolide B Related Classifications

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