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  6. (+)-Intermedine

(+)-Intermedine, a pyrrolizidine alkaloid (PA), exhibits significant cytotoxicity in neural progenitor cells (NPCs).

For research use only. We do not sell to patients.

(+)-Intermedine Chemical Structure

(+)-Intermedine Chemical Structure

CAS No. : 10285-06-0

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1 mg USD 600 In-stock
5 mg USD 1800 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of (+)-Intermedine:

(+)-Intermedine Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

(+)-Intermedine, a pyrrolizidine alkaloid (PA), exhibits significant cytotoxicity in neural progenitor cells (NPCs)[1].

In Vitro

(+)-Intermedine (1.1-30 μM; 24 hours) exhibits significant cytotoxicity at 30 μM and reduces cell viability in a concentration dependent manner in neural progenitor cells (NPCs)[1].
(+)-Intermedine showsno differentiation impairment[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

(+)-Intermedine Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: Neural progenitor cells (NPCs)
Concentration: 1.1, 3.3, 10, 30 μM
Incubation Time: 24 hours
Result: Exhibited significant cytotoxicity at 30 μM and reduced cell viability in a concentration dependent manner.
Molecular Weight

299.36

Formula

C15H25NO5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(C)[C@@]([C@H](O)C)(O)C(OCC1=CCN2[C@@]1([H])[C@H](O)CC2)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (167.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3405 mL 16.7023 mL 33.4046 mL
5 mM 0.6681 mL 3.3405 mL 6.6809 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (4.18 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 1.25 mg/mL (4.18 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥99.0%

COA (252 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3405 mL 16.7023 mL 33.4046 mL 83.5115 mL
5 mM 0.6681 mL 3.3405 mL 6.6809 mL 16.7023 mL
10 mM 0.3340 mL 1.6702 mL 3.3405 mL 8.3511 mL
15 mM 0.2227 mL 1.1135 mL 2.2270 mL 5.5674 mL
20 mM 0.1670 mL 0.8351 mL 1.6702 mL 4.1756 mL
25 mM 0.1336 mL 0.6681 mL 1.3362 mL 3.3405 mL
30 mM 0.1113 mL 0.5567 mL 1.1135 mL 2.7837 mL
40 mM 0.0835 mL 0.4176 mL 0.8351 mL 2.0878 mL
50 mM 0.0668 mL 0.3340 mL 0.6681 mL 1.6702 mL
60 mM 0.0557 mL 0.2784 mL 0.5567 mL 1.3919 mL
80 mM 0.0418 mL 0.2088 mL 0.4176 mL 1.0439 mL
100 mM 0.0334 mL 0.1670 mL 0.3340 mL 0.8351 mL
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(+)-Intermedine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(+)-Intermedine10285-06-0OtherspyrrolizidinealkaloidneuralprogenitorcellsNPCsInhibitorinhibitorinhibit

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