2. Apoptosis Stem Cell/Wnt Anti-infection
  3. Bacterial Apoptosis Wnt Fungal Parasite
  4. Prodigiosin

Prodigiosin  (Synonyms: Prodigiosine; NSC47147)

Cat. No.: HY-100711 Purity: 99.89%
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Prodigiosin (Prodigiosine) is a red pigment produced by bacteria as a bioactive secondary metabolite. Prodigiosin is a potent inhibitor of the Wnt/β-catenin pathway. Prodigiosin has antibacterial, antifungal, antiprotozoal, antimalarial, immunosuppressive, and anticancer properties.

For research use only. We do not sell to patients.

Prodigiosin Chemical Structure

Prodigiosin Chemical Structure

CAS No. : 82-89-3

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100 μg USD 50 In-stock
250 μg USD 90 In-stock
500 μg USD 150 In-stock
1 mg USD 240 In-stock
5 mg USD 850 In-stock
10 mg USD 1360 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Prodigiosin:

Prodigiosin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Prodigiosin

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Prodigiosin (Prodigiosine) is a red pigment produced by bacteria as a bioactive secondary metabolite. Prodigiosin is a potent inhibitor of the Wnt/β-catenin pathway. Prodigiosin has antibacterial, antifungal, antiprotozoal, antimalarial, immunosuppressive, and anticancer properties[1][2].

IC50 & Target

Plasmodium

 

In Vitro

Prodigiosin (25-500 nM; 24 hours) treatment reduces the viability of breast cancer cells, with IC50 values at 48 h of 62.52 nM in MDA-MB-231 cells and 261.2 nM in MDA-MB-468 cells[1].
Prodigiosin (25-500 nM; 24 hours) treatment significantly reduces the levels of phosphorylated LRP6 and DVL2, active β-catenin, and total β-catenin. Prodigiosin noticeably inhibits the phosphorylation of GSK3β at Ser9 in HEK293T cells, which is indicative of an increase in GSK3β activity[1].
Prodigiosin can inhibit proliferation and induce apoptosis in breast cancer cells[1].
Prodigiosin (25-500 nM; 24 hours) treatment dose-dependently blocks Wnt signaling activated by Wnt1, Wnt3, Wnt1/LRP6, Wnt3/LRP6, and Dishevelled 2 (DVL2) in transfected HEK293T cells. Prodigiosin treatment inhibits Wnt3A-CM-induced transcription in a dose-dependent manner. Prodigiosin inhibits transcription of the SuperTopFlash reporter activated by either Wnt transfection or Wnt3A treatment[1].
When applied to cultures of chytrid fungi Batrachochytrium dendrobatidis and B. salamandrivorans, Prodigiosin causes significant growth inhibition, with MIC values of 10 μM and 50 μM, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Prodigiosin Related Antibodies

Cell Proliferation Assay[1]

Cell Line: MDA-MB-231 and MDA-MB-468 cells
Concentration: 10 nM, 25 nM, 50 nM, 100 nM, 250 nM, 500 nM, 1000 nM, 2500 nM, 5000 nM
Incubation Time: 24 hours, 48 hours
Result: Reduced the viability of breast cancer cells, with IC50 values at 48 h of 62.52 nM in MDA-MB-231 cells and 261.2 nM in MDA-MB-468 cells.

Western Blot Analysis[1]

Cell Line: HEK293T cells
Concentration: 50 nM, 100 nM, 250 nM, 500 nM
Incubation Time: 24 hours
Result: Significantly reduced the levels of phosphorylated LRP6 and DVL2, active β-catenin, and total β-catenin.
In Vivo

Prodigiosin (5 mg/kg; intraperitoneal injection; twice weekly; for 3 weeks) treatment significantly inhibits tumor growth. Prodigiosin treatment decreases tumor cell density and expression of the proliferation marker Ki-67[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c nude mice injected with MDA-MB-231 cells[1]
Dosage: 5 mg/kg
Administration: Intraperitoneal injection; twice weekly; for 3 weeks
Result: Significantly inhibited tumor growth in mice.
Molecular Weight

323.43

Formula

C20H25N3O
CAS No.
Appearance

Solid

Color

Brown to red

SMILES

CCCCCC1=C/C(N=C1C)=C\C2=C(OC)C=C(C3=CC=CN3)N2
Structure Classification
Initial Source

Serratia marcescens
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (77.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0919 mL 15.4593 mL 30.9186 mL
5 mM 0.6184 mL 3.0919 mL 6.1837 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  2% DMSO    40% PEG300    5% Tween-80    53% Saline

    Solubility: ≥ 0.5 mg/mL (1.55 mM); Clear solution

  • Protocol 2

    Add each solvent one by one:  2% DMSO    98% (20% SBE-β-CD in Saline)

    Solubility: 0.5 mg/mL (1.55 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.89%

COA (248 KB) RP-HPLC (225 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0919 mL 15.4593 mL 30.9186 mL 77.2965 mL
5 mM 0.6184 mL 3.0919 mL 6.1837 mL 15.4593 mL
10 mM 0.3092 mL 1.5459 mL 3.0919 mL 7.7296 mL
15 mM 0.2061 mL 1.0306 mL 2.0612 mL 5.1531 mL
20 mM 0.1546 mL 0.7730 mL 1.5459 mL 3.8648 mL
25 mM 0.1237 mL 0.6184 mL 1.2367 mL 3.0919 mL
30 mM 0.1031 mL 0.5153 mL 1.0306 mL 2.5765 mL
40 mM 0.0773 mL 0.3865 mL 0.7730 mL 1.9324 mL
50 mM 0.0618 mL 0.3092 mL 0.6184 mL 1.5459 mL
60 mM 0.0515 mL 0.2577 mL 0.5153 mL 1.2883 mL
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Prodigiosin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Prodigiosin82-89-3Prodigiosine NSC47147NSC47147NSC 47147NSC-47147BacterialApoptosisWntFungalParasiteAntibacterialantifungalantiprotozoalantimalarialimmunosuppressiveanticancerpigmentβ-cateninInhibitorinhibitorinhibit

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