2. Metabolic Enzyme/Protease
  3. Prolyl Endopeptidase (PREP)
  4. Prolyl Endopeptidase Inhibitor 1

Prolyl Endopeptidase Inhibitor 1  (Synonyms: Boc-Pro-prolinal; (Boc)-Prolyl-prolinal)

Cat. No.: HY-113951 Purity: 98.34%
COA Handling Instructions

Molarity Calculator

Prolyl Endopeptidase Inhibitor 1 (Boc-Pro-prolinal) is a potent prolyl endopeptidase (PEP; PE) inhibitor, with a Ki value of 15 nM. Prolyl Endopeptidase Inhibitor 1 has anti-amnesic effect.

For research use only. We do not sell to patients.

Prolyl Endopeptidase Inhibitor 1 Chemical Structure

Prolyl Endopeptidase Inhibitor 1 Chemical Structure

CAS No. : 86925-97-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 424 In-stock
Solution
10 mM * 1 mL in DMSO USD 424 In-stock
Solid
1 mg USD 130 In-stock
5 mg USD 290 In-stock
10 mg USD 460 In-stock
25 mg USD 890 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Prolyl Endopeptidase Inhibitor 1 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

Prolyl Endopeptidase Inhibitor 1 (Boc-Pro-prolinal) is a potent prolyl endopeptidase (PEP; PE) inhibitor, with a Ki value of 15 nM. Prolyl Endopeptidase Inhibitor 1 has anti-amnesic effect[1].

IC50 & Target

Ki: 15 nM (prolyl endopeptidase)[1]
In Vitro

Prolyl Endopeptidase Inhibitor 1 (Boc-Pro-prolinal) strongly inhibits the mammalian prolyl endopeptidase with a Ki value of nM order, while the Ki value for the microbial enzyme is only of microM order (Ki=3.2 μM for Flavobacterium)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Prolyl Endopeptidase Inhibitor 1 Related Antibodies
Molecular Weight

296.36

Formula

C15H24N2O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N1[C@H](C(N2[C@H](C=O)CCC2)=O)CCC1)OC(C)(C)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (337.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3743 mL 16.8714 mL 33.7427 mL
5 mM 0.6749 mL 3.3743 mL 6.7485 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.44 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.44 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.34%

COA (252 KB) HNMR (267 KB) RP-HPLC (243 KB) MS (73 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3743 mL 16.8714 mL 33.7427 mL 84.3569 mL
5 mM 0.6749 mL 3.3743 mL 6.7485 mL 16.8714 mL
10 mM 0.3374 mL 1.6871 mL 3.3743 mL 8.4357 mL
15 mM 0.2250 mL 1.1248 mL 2.2495 mL 5.6238 mL
20 mM 0.1687 mL 0.8436 mL 1.6871 mL 4.2178 mL
25 mM 0.1350 mL 0.6749 mL 1.3497 mL 3.3743 mL
30 mM 0.1125 mL 0.5624 mL 1.1248 mL 2.8119 mL
40 mM 0.0844 mL 0.4218 mL 0.8436 mL 2.1089 mL
50 mM 0.0675 mL 0.3374 mL 0.6749 mL 1.6871 mL
60 mM 0.0562 mL 0.2812 mL 0.5624 mL 1.4059 mL
80 mM 0.0422 mL 0.2109 mL 0.4218 mL 1.0545 mL
100 mM 0.0337 mL 0.1687 mL 0.3374 mL 0.8436 mL
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Prolyl Endopeptidase Inhibitor 1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Prolyl Endopeptidase Inhibitor 186925-97-5Boc-Pro-prolinal (Boc)-Prolyl-prolinalProlyl Endopeptidase Inhibitor1Prolyl Endopeptidase Inhibitor-1Prolyl Endopeptidase (PREP)prolyl oligopeptidasePOPprolylendopeptidaseanti-amnesicmammalianenzymeInhibitorinhibitorinhibit

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