PROTAC BRD4 ligand-2 hydrochloride

Name: PROTAC BRD4 ligand-2 hydrochloride
Brand: MedChemExpress
SKU: HY-132942A
Rating: 4.5 (1 reviews)

Cat. No.: HY-132942A Purity: 99.65%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

PROTAC BRD4 ligand-2 hydrochloride is a ligand for target BRD4 protein for PROTAC CFT-2718.

For research use only. We do not sell to patients.

PROTAC BRD4 ligand-2 hydrochloride Chemical Structure

Size	Price	Stock	Quantity
1 mg	USD 482	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 1060	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1690	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of PROTAC BRD4 ligand-2 hydrochloride:

PROTAC BRD4 ligand-2 Get quote

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

PROTAC BRD4 ligand-2 hydrochloride is a ligand for target BRD4 protein for PROTAC CFT-2718.

Molecular Weight

437.32

Formula

C₂₂H₁₈Cl₂N₆

Appearance

Solid

Color

Off-white to light yellow

SMILES

ClC1=CC=C(C2=NC3(CC3)C4=NN=C(C)N4C5=CC=C(C6=CNN=C6)C=C25)C=C1.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (228.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2867 mL	11.4333 mL	22.8666 mL
5 mM	0.4573 mL	2.2867 mL	4.5733 mL
10 mM	0.2287 mL	1.1433 mL	2.2867 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.72 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.65%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (257 KB) HNMR (308 KB) LCMS (137 KB)

Handling Instructions (2659 KB)

References

[1]. Sun D, et al. Evaluation of the Small-molecule BRD4 Degrader CFT-2718 in Small-cell Lung Cancer and Pancreatic Cancer Models. Mol Cancer Ther. 2021 Aug;20(8):1367-1377. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2867 mL	11.4333 mL	22.8666 mL	57.1664 mL
	5 mM	0.4573 mL	2.2867 mL	4.5733 mL	11.4333 mL
	10 mM	0.2287 mL	1.1433 mL	2.2867 mL	5.7166 mL
	15 mM	0.1524 mL	0.7622 mL	1.5244 mL	3.8111 mL
	20 mM	0.1143 mL	0.5717 mL	1.1433 mL	2.8583 mL
	25 mM	0.0915 mL	0.4573 mL	0.9147 mL	2.2867 mL
	30 mM	0.0762 mL	0.3811 mL	0.7622 mL	1.9055 mL
	40 mM	0.0572 mL	0.2858 mL	0.5717 mL	1.4292 mL
	50 mM	0.0457 mL	0.2287 mL	0.4573 mL	1.1433 mL
	60 mM	0.0381 mL	0.1906 mL	0.3811 mL	0.9528 mL
	80 mM	0.0286 mL	0.1429 mL	0.2858 mL	0.7146 mL
	100 mM	0.0229 mL	0.1143 mL	0.2287 mL	0.5717 mL