2. GPCR/G Protein
  3. Protease Activated Receptor (PAR)
  4. Protease-Activated Receptor-1, PAR-1 Agonist TFA

Protease-Activated Receptor-1, PAR-1 Agonist TFA 

Cat. No.: HY-P2518A Purity: 98.54%
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Protease-Activated Receptor-1, PAR-1 Agonist TFA is a selective proteinase-activated receptor1 (PAR-1) agonist peptide. Protease-Activated Receptor-1, PAR-1 Agonist TFA corresponds to PAR1 tethered ligand and which can selectively mimic theactions of thrombin via this receptor.

For research use only. We do not sell to patients.

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Protease-Activated Receptor-1, PAR-1 Agonist TFA Chemical Structure

Protease-Activated Receptor-1, PAR-1 Agonist TFA Chemical Structure

Size Price Stock Quantity
1 mg USD 50 In-stock
5 mg USD 125 In-stock
10 mg USD 190 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Protease-Activated Receptor-1, PAR-1 Agonist TFA:

Protease-Activated Receptor-1, PAR-1 Agonist TFA Related Antibodies

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PAR1 PAR2 PAR4

  • Biological Activity

  • Purity & Documentation

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Description

Protease-Activated Receptor-1, PAR-1 Agonist TFA is a selective proteinase-activated receptor1 (PAR-1) agonist peptide. Protease-Activated Receptor-1, PAR-1 Agonist TFA corresponds to PAR1 tethered ligand and which can selectively mimic theactions of thrombin via this receptor[1][2].

IC50 & Target

PAR1

 

In Vitro

Protease-Activated Receptor-1, PAR-1 Agonist induces activation of protein kinase C isoenzymes alpha and epsilon in human HT-29 colon carcinoma cells expressing PAR1 endogeneously. On the cellular level, Protease-Activated Receptor-1, PAR-1 Agonist and thrombin prompted HT-29 cell migration and matrix adhesion by a PKCepsilon-dependent mechanism as concluded because of the inhibition of PAR1-mediated effects by the PKC inhibitor bisindolylmaleimide I and the PKCepsilon translocation inhibitory peptide EAVSLKPT but not by the PKC inhibitor G? 6976[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Protease-Activated Receptor-1, PAR-1 Agonist TFA Related Antibodies
Molecular Weight

876.92

Formula

C37H59F3N10O11
Appearance

Solid

Color

White to off-white

Sequence

Thr-Phe-Leu-Leu-Arg-Asn
Sequence Shortening

TFLLRN
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 6.67 mg/mL (7.61 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1404 mL 5.7018 mL 11.4035 mL
5 mM 0.2281 mL 1.1404 mL 2.2807 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 5 mg/mL (5.70 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.1404 mL 5.7018 mL 11.4035 mL 28.5089 mL
5 mM 0.2281 mL 1.1404 mL 2.2807 mL 5.7018 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Protease-Activated Receptor-1, PAR-1 Agonist TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Protease-Activated Receptor-1, PAR-1 AgonistProtease Activated Receptor (PAR)Thrombin receptorsmigrationmatrixadhesioncoloncarcinomacellsselectiveInhibitorinhibitorinhibit

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