Purine nucleoside phosphorylase, Microorganism  (Synonyms: PNP)

Purine nucleoside phosphorylase, Microorganism (PNP) is a key enzyme in purine metabolism, which is involved in the purine rescue pathway. The deficiency of Purine nucleoside phosphorylase resulted in impaired T cell function. In the presence of inorganic orthophosphate as the second substrate, Purine nucleoside phosphorylase catalyzes the breaking of the glycosidic bond between ribose and deoxyribonucleoside to generate purine base and ribose (deoxyribose) -1-phosphate^[1].

Product Information
Molecular weight: about 100 kDa, trimer
pH stability: 6.0-9.0, optimum pH 6.5-8.0
Inhibitors: p-Chloromercuric benzoic acid, SDS, Hg²⁺, Ag⁺

Instructions
Soluble in water (below 100 mg/mL concentration), or dissolved in buffer according to specific experimental references.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9030-21-1

Solid

White to off-white

[Purine nucleoside phosphorylase, Microorganism]

2.4.2.1

≥10 U/mg soild

One unit is defined as the amount of enzyme that will cause the phosphorolysis of 1μ mol of inosine to hypoxanthine and ribose 1-phosphate per min at pH 7.7 at 37°C.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Bzowska A, et al. Purine nucleoside phosphorylases: properties, functions, and clinical aspects. Pharmacol Ther. 2000 Dec;88(3):349-425.  [Content Brief]