Qingyangshengenin A

Name: Qingyangshengenin A
Brand: MedChemExpress
SKU: HY-N0581
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0581 Purity: 99.25%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Qingyangshengenin A, a C-21 steroidal glycoside isolated from the roots of Cynanchum otophyllum Schneid, has antiepileptic activity.

For research use only. We do not sell to patients.

Qingyangshengenin A Chemical Structure

CAS No. : 106644-33-1

Size	Price	Stock	Quantity
5 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Qingyangshengenin A, a C-21 steroidal glycoside isolated from the roots of Cynanchum otophyllum Schneid, has antiepileptic activity^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 40 μM Compound: 15	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTS assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTS assay	[PMID: 29625825]
HL-60	IC₅₀	29.1 μM Compound: 15	Cytotoxicity against human HL60 cells assessed as reduction in cell viability after 48 hrs by MTS assay Cytotoxicity against human HL60 cells assessed as reduction in cell viability after 48 hrs by MTS assay	[PMID: 29625825]
MCF7	IC₅₀	> 40 μM Compound: 15	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTS assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTS assay	[PMID: 29625825]
SMMC-7721	IC₅₀	> 40 μM Compound: 15	Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability after 48 hrs by MTS assay Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability after 48 hrs by MTS assay	[PMID: 29625825]
SW480	IC₅₀	> 40 μM Compound: 15	Cytotoxicity against human SW480 cells assessed as reduction in cell viability after 48 hrs by MTS assay Cytotoxicity against human SW480 cells assessed as reduction in cell viability after 48 hrs by MTS assay	[PMID: 29625825]

Molecular Weight

933.09

Formula

C₄₉H₇₂O₁₇

CAS No.

106644-33-1

Appearance

Solid

SMILES

C[C@@]([C@@H](C[C@]1([H])[C@]23C)OC(C4=CC=C(O)C=C4)=O)([C@]5(O)C(C)=O)[C@@]([C@@]1(CC=C2C[C@@H](O[C@@](O[C@H](C)[C@H]6O[C@@](O[C@H](C)[C@H]7O[C@@](O[C@H](C)[C@H]8O)([H])C[C@H]8OC)([H])C[C@@H]7OC)([H])C[C@@H]6OC)CC3)O)(CC5)O

Structure Classification

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (107.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0717 mL	5.3585 mL	10.7171 mL
5 mM	0.2143 mL	1.0717 mL	2.1434 mL
10 mM	0.1072 mL	0.5359 mL	1.0717 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.25%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (253 KB) HNMR (297 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Mu QZ, et al. Two new antiepilepsy compounds--otophyllosides A and B. Sci Sin B. 1986 Mar;29(3):295-301. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.0717 mL	5.3585 mL	10.7171 mL	26.7927 mL
	5 mM	0.2143 mL	1.0717 mL	2.1434 mL	5.3585 mL
	10 mM	0.1072 mL	0.5359 mL	1.0717 mL	2.6793 mL
	15 mM	0.0714 mL	0.3572 mL	0.7145 mL	1.7862 mL
	20 mM	0.0536 mL	0.2679 mL	0.5359 mL	1.3396 mL
	25 mM	0.0429 mL	0.2143 mL	0.4287 mL	1.0717 mL
	30 mM	0.0357 mL	0.1786 mL	0.3572 mL	0.8931 mL
	40 mM	0.0268 mL	0.1340 mL	0.2679 mL	0.6698 mL
	50 mM	0.0214 mL	0.1072 mL	0.2143 mL	0.5359 mL
	60 mM	0.0179 mL	0.0893 mL	0.1786 mL	0.4465 mL
	80 mM	0.0134 mL	0.0670 mL	0.1340 mL	0.3349 mL
	100 mM	0.0107 mL	0.0536 mL	0.1072 mL	0.2679 mL