QX-314 chloride

Name: QX-314 chloride
Brand: MedChemExpress
SKU: HY-108505
Rating: 4.5 (1 reviews)

Cat. No.: HY-108505 Purity: 99.83%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

QX-314 chloride is a membrane-impermeable permanently charged sodium channel blocker.

For research use only. We do not sell to patients.

QX-314 chloride Chemical Structure

CAS No. : 5369-03-9

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of QX-314 chloride:

QX-314 bromide Get quote

1 Publications Citing Use of MCE QX-314 chloride

•Immunity. 2024 Mar 12;57(3):495-512.e11. [Abstract]

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View All Sodium Channel Isoform Specific Products:

View All Isoforms

Na_v1.1 Na_v1.2 Na_v1.3 Na_v1.4 Na_v1.5 Na_v1.6 Na_v1.7 Na_v1.8

Biological Activity
Purity & Documentation
References
Customer Review

Description

QX-314 chloride is a membrane-impermeable permanently charged sodium channel blocker^[1].

IC₅₀ & Target

sodium channel^[1]

In Vitro

QX-314 chloride exerts biphasic effects on transient receptor potential vanilloid subtype 1 channels (TRPV1) in vitro^[2].
QX-314 chloride (1–60 mM) directly activates TRPV1 in a concentration-dependent manner^[2].
QX-314 chloride (10 mM) inhibits calcium currents in hippocampal CA1 pyramidal neurons intracellular, and the low-threshold (T-type) Ca²⁺ currents are on average < 45% of control amplitude^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

298.85

Formula

C₁₆H₂₇ClN₂O

CAS No.

5369-03-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=C(C)C=CC=C1C)C[N+](CC)(CC)CC.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (167.31 mM; Need ultrasonic)

DMSO : 12.5 mg/mL (41.83 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3462 mL	16.7308 mL	33.4616 mL
5 mM	0.6692 mL	3.3462 mL	6.6923 mL
10 mM	0.3346 mL	1.6731 mL	3.3462 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.14 mg/mL (3.81 mM); Clear solution

This protocol yields a clear solution of ≥ 1.14 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.4 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.83 mg/mL (2.78 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.83 mg/mL (2.78 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (334.62 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (249 KB) HNMR (175 KB) LCMS (112 KB)

Handling Instructions (2659 KB)

References

[1]. Rivera-Acevedo RE, et al. The quaternary lidocaine derivative, QX-314, exerts biphasic effects on transient receptor potential vanilloid subtype 1 channels in vitro. Anesthesiology. 2011 Jun;114(6):1425-34. [Content Brief]

[2]. Talbot MJ, et al. Intracellular QX-314 inhibits calcium currents in hippocampal CA1 pyramidal neurons. J Neurophysiol. 1996 Sep;76(3):2120-4. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.3462 mL	16.7308 mL	33.4616 mL	83.6540 mL
	5 mM	0.6692 mL	3.3462 mL	6.6923 mL	16.7308 mL
	10 mM	0.3346 mL	1.6731 mL	3.3462 mL	8.3654 mL
	15 mM	0.2231 mL	1.1154 mL	2.2308 mL	5.5769 mL
	20 mM	0.1673 mL	0.8365 mL	1.6731 mL	4.1827 mL
	25 mM	0.1338 mL	0.6692 mL	1.3385 mL	3.3462 mL
	30 mM	0.1115 mL	0.5577 mL	1.1154 mL	2.7885 mL
	40 mM	0.0837 mL	0.4183 mL	0.8365 mL	2.0914 mL
H₂O	50 mM	0.0669 mL	0.3346 mL	0.6692 mL	1.6731 mL
	60 mM	0.0558 mL	0.2788 mL	0.5577 mL	1.3942 mL
	80 mM	0.0418 mL	0.2091 mL	0.4183 mL	1.0457 mL
	100 mM	0.0335 mL	0.1673 mL	0.3346 mL	0.8365 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

QX-314 chloride Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

QX-3145369-03-9QX314QX 314Sodium ChannelNa channelsNa+ channelssodiumchannelcalciumInhibitorinhibitorinhibit

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QX-314 chloride

Customer Review

Other Forms of QX-314 chloride:

QX-314 chloride Related Antibodies

1 Publications Citing Use of MCE QX-314 chloride

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Sodium Channel Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

QX-314 chloride Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

QX-314 chloride Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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