2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite Drug Metabolite
  4. (±)-Nornicotine

(±)-Nornicotine  (Synonyms: Nornicotine)

Cat. No.: HY-W002112 Purity: ≥96.0%
COA Handling Instructions

Molarity Calculator

(±)-Nornicotine is the primary metabolite of nicotine that is formed through demethylation of nicotine in the genus Nicotiana tabacum L. (±)-Nornicotine is a precursor of tobacco-specific nitrosamine N-nitrosonornicotine. (±)-Nornicotine has detrimental effects to human health.

For research use only. We do not sell to patients.

(±)-Nornicotine Chemical Structure

(±)-Nornicotine Chemical Structure

CAS No. : 5746-86-1

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100 mg USD 75 In-stock
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Based on 1 publication(s) in Google Scholar

(±)-Nornicotine Related Antibodies

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Description

(±)-Nornicotine is the primary metabolite of nicotine that is formed through demethylation of nicotine in the genus Nicotiana tabacum L. (±)-Nornicotine is a precursor of tobacco-specific nitrosamine N-nitrosonornicotine. (±)-Nornicotine has detrimental effects to human health[1].

Molecular Weight

148.21

Formula

C9H12N2
CAS No.
Appearance

Liquid (Density: 1.042±0.06 g/cm3)

Color

Colorless to light yellow

SMILES

C1(C2CCCN2)=CN=CC=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (674.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.7472 mL 33.7359 mL 67.4718 mL
5 mM 1.3494 mL 6.7472 mL 13.4944 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (16.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (16.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥97.0%

COA (252 KB) HNMR (121 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.7472 mL 33.7359 mL 67.4718 mL 168.6796 mL
5 mM 1.3494 mL 6.7472 mL 13.4944 mL 33.7359 mL
10 mM 0.6747 mL 3.3736 mL 6.7472 mL 16.8680 mL
15 mM 0.4498 mL 2.2491 mL 4.4981 mL 11.2453 mL
20 mM 0.3374 mL 1.6868 mL 3.3736 mL 8.4340 mL
25 mM 0.2699 mL 1.3494 mL 2.6989 mL 6.7472 mL
30 mM 0.2249 mL 1.1245 mL 2.2491 mL 5.6227 mL
40 mM 0.1687 mL 0.8434 mL 1.6868 mL 4.2170 mL
50 mM 0.1349 mL 0.6747 mL 1.3494 mL 3.3736 mL
60 mM 0.1125 mL 0.5623 mL 1.1245 mL 2.8113 mL
80 mM 0.0843 mL 0.4217 mL 0.8434 mL 2.1085 mL
100 mM 0.0675 mL 0.3374 mL 0.6747 mL 1.6868 mL
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(±)-Nornicotine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(±)-Nornicotine5746-86-1NornicotineEndogenous MetaboliteDrug Metabolitenicotinemetaboliteprecursornicotiana tabacum Ldetrimentaltobacco-specificnitrosamineInhibitorinhibitorinhibit

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