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  4. ABCA1 Protein, Human (HEK293, His, Strep, FLAG)

ABCA1 Protein, Human (HEK293, His, Strep, FLAG)

Cat. No.: HY-P701357
COA Handling Instructions

The ABCA1 protein catalyzes the translocation of specific phospholipids from the cytoplasm to the extracellular/luminal membrane leaflet in response to ATP hydrolysis. It achieves phospholipid translocation by forcing phospholipids through its channels and loops into elongated hydrophobic tunnels in its extracellular domain. ABCA1 Protein, Human (HEK293, His, Strep, FLAG) is the recombinant human-derived ABCA1 protein, expressed by HEK293 , with N-Strep, C-Flag, N-8*His labeled tag. The total length of ABCA1 Protein, Human (HEK293, His, Strep, FLAG) is 2260 a.a., .

For research use only. We do not sell to patients.

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10 μg $805 In-stock
50 μg $2250 In-stock
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  • Biological Activity

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Description

The ABCA1 protein catalyzes the translocation of specific phospholipids from the cytoplasm to the extracellular/luminal membrane leaflet in response to ATP hydrolysis. It achieves phospholipid translocation by forcing phospholipids through its channels and loops into elongated hydrophobic tunnels in its extracellular domain. ABCA1 Protein, Human (HEK293, His, Strep, FLAG) is the recombinant human-derived ABCA1 protein, expressed by HEK293 , with N-Strep, C-Flag, N-8*His labeled tag. The total length of ABCA1 Protein, Human (HEK293, His, Strep, FLAG) is 2260 a.a., .

Background

ABCA1 protein functions as a crucial catalyst in the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of the membrane, a process driven by ATP hydrolysis. This activity is integral to the transfer of phospholipids to apolipoproteins, leading to the formation of nascent high-density lipoproteins (HDLs). ABCA1 exhibits a preference for transporting phosphatidylcholine over phosphatidylserine and may play a similar role in the efflux of intracellular cholesterol to apolipoproteins, contributing to the generation of nascent HDLs. The mechanism involves the translocation of phospholipids from the outer face of the plasma membrane, forcing them through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain. This intricate process is essential for the regulation of lipid homeostasis and the maintenance of cellular and systemic lipid balance.

Species

Human

Source

HEK293

Tag

N-Strep;C-Flag;N-8*His

Accession

O95477 (A2-V2261)

Gene ID

19

Molecular Construction
N-term
8*His-Strep
ABCA1 (A2-V2261)
Accession # O95477
Flag
C-term
Synonyms
ABCA1; ABC1; CERP
Molecular Weight

Approximately 258.4 KDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM HEPES pH 7.5, 150 mM NaCl, 2 mM DTT, cOmplete Protease Inhibitor Tablet (Roche), 0.005% (w/v) GDN.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

ABCA1 Protein, Human (HEK293, His, Strep, FLAG) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
ABCA1 Protein, Human (HEK293, His, Strep, FLAG)
Cat. No.:
HY-P701357
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