1. Recombinant Proteins
  2. Cytokines and Growth Factors Animal-free Recombinant Proteins
  3. Interleukin & Receptors
  4. IL-27
  5. IL-27 beta/EBI3
  6. Animal-Free IL-27 beta/EBI3 Protein, Human (His)

Animal-Free IL-27 beta/EBI3 Protein, Human (His)

Cat. No.: HY-P700118AF
SDS COA Handling Instructions

The IL-27 beta/EBI3 protein binds to IL27 to form IL-27 interleukin, which is critical in innate immunity. IL-27 has both pro- and anti-inflammatory properties, regulating T helper cell development, inhibiting T cell proliferation, stimulating cytotoxic T cell activity, inducing B cell isotype switching, and affecting innate immune cells. Animal-Free IL-27 beta/EBI3 Protein, Human (His) is the recombinant human-derived animal-FreeIL-27 beta/EBI3 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-27 beta/EBI3 Protein, Human (His) is 209 a.a., with molecular weight of ~24.25 kDa.

Animal-free recombinant proteins offer high consistency and stability, whithout using or contacting of any animal-derived materials and reagents.

For research use only. We do not sell to patients.

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Description

The IL-27 beta/EBI3 protein binds to IL27 to form IL-27 interleukin, which is critical in innate immunity. IL-27 has both pro- and anti-inflammatory properties, regulating T helper cell development, inhibiting T cell proliferation, stimulating cytotoxic T cell activity, inducing B cell isotype switching, and affecting innate immune cells. Animal-Free IL-27 beta/EBI3 Protein, Human (His) is the recombinant human-derived animal-FreeIL-27 beta/EBI3 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-27 beta/EBI3 Protein, Human (His) is 209 a.a., with molecular weight of ~24.25 kDa.

Background

The IL-27 beta/EBI3 Protein associates with IL27 to constitute the IL-27 interleukin, a heterodimeric cytokine that plays a crucial role in innate immunity. Demonstrating both pro- and anti-inflammatory properties, IL-27 regulates T-helper cell development, inhibits T-cell proliferation, stimulates cytotoxic T-cell activity, induces isotype switching in B-cells, and exerts diverse effects on innate immune cells. CD4 T-helper cells, under IL-27 influence, can differentiate into type 1 effector cells (TH1), type 2 effector cells (TH2), and IL-17-producing helper T-cells (TH17). IL-27 facilitates the rapid clonal expansion of naive CD4 T-cells, but not memory CD4 T-cells, and synergizes strongly with IL-12 to induce interferon-gamma/IFN-gamma production by naive CD4 T-cells. Binding to the cytokine receptor WSX-1/TCCR, IL-27 contributes to the antitumor and antiangiogenic activities, leading to the activation of production of antiangiogenic chemokines. It forms heterodimers with IL27/IL27A, recognized as interleukin IL-27, and with IL12A, forming interleukin IL-35, both not disulfide-linked. Additionally, IL-27 interacts with SQSTM1.

Biological Activity

Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is <2 ng/mL.

Species

Human

Source

E. coli

Tag

C-His

Accession

Q14213 (R21-K229)

Gene ID
Molecular Construction
N-term
IL-27B (R21-K229)
Accession # Q14213
His
C-term
Synonyms
Interleukin-27 subunit alpha; IL-27-A; Interleukin-27 subunit beta; IL-27B; Epstein-Barr virus-in_x0002_duced gene 3 protein; EBV-induced gene 3 protein; EBI3; p28; Interleukin-30; IL-30
AA Sequence

MRKGPPAALTLPRVQCRASRYPIAVDCSWTLPPAPNSTSPVSFIATYRLGMAARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVTAVHPWGSSSSFVPFITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYKRQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLPATATMSLGK

Molecular Weight

Approximately 24.25 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

Animal-Free IL-27 beta/EBI3 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Animal-Free IL-27 beta/EBI3 Protein, Human (His)
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