1. Recombinant Proteins
  2. Cytokines and Growth Factors Animal-free Recombinant Proteins
  3. Interleukin & Receptors
  4. IL-36
  5. IL-36 gamma
  6. Animal-Free IL-36 gamma/IL-1F9 Protein, Mouse (His)

Animal-Free IL-36 gamma/IL-1F9 Protein, Mouse (His)

Cat. No.: HY-P700211AF
SDS COA Handling Instructions

IL-36 gamma/IL-1F9 protein activates NF-κB through IL1RL2 and promotes local inflammatory responses in the epithelial barrier. It affects keratinocytes, dendritic cells and T cells, promoting tissue infiltration, cell maturation and proliferation. Animal-Free IL-36 gamma/IL-1F9 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-36 gamma/IL-1F9 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-36 gamma/IL-1F9 Protein, Mouse (His) is 152 a.a., with molecular weight of ~18.27 kDa.

Animal-free recombinant proteins offer high consistency and stability, whithout using or contacting of any animal-derived materials and reagents.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
Free Sample   Apply now
2 μg In-stock
10 μg In-stock
50 μg In-stock
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

IL-36 gamma/IL-1F9 protein activates NF-κB through IL1RL2 and promotes local inflammatory responses in the epithelial barrier. It affects keratinocytes, dendritic cells and T cells, promoting tissue infiltration, cell maturation and proliferation. Animal-Free IL-36 gamma/IL-1F9 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-36 gamma/IL-1F9 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-36 gamma/IL-1F9 Protein, Mouse (His) is 152 a.a., with molecular weight of ~18.27 kDa.

Background

IL-36 gamma/IL-1F9 Protein acts as an agonist, activating NF-kappa B through the orphan IL-1-receptor-related protein 2/IL1RL2. As part of the IL-36 signaling system, it is believed to be present in epithelial barriers, contributing to local inflammatory responses, akin to the IL-1 system, sharing the coreceptor IL1RAP. This protein is implicated in skin inflammatory responses, influencing keratinocytes, dendritic cells, and, indirectly, T-cells to drive tissue infiltration, cell maturation, and proliferation. Additionally, it may play a role in pro-inflammatory responses during specific neutrophilic airway inflammations and contribute to the innate immune response against fungal pathogens. IL-36 gamma induces the production of various pro-inflammatory cytokines in bone marrow-derived dendritic cells (BMDCs) and enhances dendritic cell maturation by stimulating the surface expression of CD80, CD86, and MHC class II. Furthermore, it induces the production of IFN-gamma, IL-4, and IL-17 in cultured CD4(+) T-cells and splenocytes. The protein interacts with the cargo receptor TMED10, mediating translocation from the cytoplasm into the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and subsequent secretion.

Biological Activity

Measure by its ability to induce IL-6 secretion in 3T3 cells. The ED50 for this effect is <15 ng/mL. The specific activity of recombinant mouse IL-36 gamma is >6 x104 IU/mg

Species

Mouse

Source

E. coli

Tag

C-His

Accession

Q8R460 (G13-S164)

Gene ID
Molecular Construction
N-term
IL-36γ (G13-S164)
Accession # Q8R460
His
C-term
Synonyms
Interleukin-36 gamma; IL-36γ; IL36G; IL-1F9; IL-1H1
AA Sequence

MGRETPDFGEVFDLDQQVWIFRNQALVTVPRSHRVTPVSVTILPCKYPESLEQDKGIAIYLGIQNPDKCLFCKEVNGHPTLLLKEEKILDLYHHPEPMKPFLFYHTRTGGTSTFESVAFPGHYIASSKTGNPIFLTSKKGEYYNINFNLDIKS

Molecular Weight

Approximately 18.27 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Animal-Free IL-36 gamma/IL-1F9 Protein, Mouse (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Animal-Free IL-36 gamma/IL-1F9 Protein, Mouse (His)
Cat. No.:
HY-P700211AF
Quantity:
MCE Japan Authorized Agent: