Annexin A3/ANXA3 Protein, Human

Annexin A3 Protein, Human is a 37 kDa endogenous and secretory multifunctional protein which belongs to the annexin family. Annexin A13 is highly expressed in various carcinomas cells. Human Annexin A3 can promote aggressive cancer and stem cell-like properties and can be used as a useful predictive biomarker in HCC^[1].

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins.
Annexin A3, also known as Annexin III and Lipocortin III (LPC3), is a calcium-dependent phospholipid-binding protein being preferentially located on the cytosolic face of the plasma membrane. Human Annexin A3 shares 89% and 85% aa sequence identity with mouse and rat Annexin A3, respectively^[1][2].

Human

E. coli

Tag Free

P12429 (M1-D323)

306  [NCBI]

MASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIGTDEKMLISILTERSNAQRQLIVKEYQAAYGKELKDDLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKGAGTNEDALIEILTTRTSRQMKDISQAYYTVYKKSLGDDISSETSGDFRKALLTLADGRRDESLKVDEHLAKQDAQILYKAGENRWGTDEDKFTEILCLRSFPQLKLTFDEYRNISQKDIVDSIKGELSGHFEDLLLAIVNCVRNTPAFLAERLHRALKGIGTDEFTLNRIMVSRSEIDLLDIRTEFKKHYGYSLYSAIKSDTSGDYEITLLKICGGDD

Approximately 33.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Man Tong, et al. Efficacy of annexin A3 blockade in sensitizing hepatocellular carcinoma to sorafenib and regorafenib. J Hepatol. 2018 Oct;69(4):826-839.  [Content Brief]

  [2]. Juan Wang, et al. Annexin A3 may play an important role in ochratoxin-induced malignant transformation of human gastric epithelium cells. Toxicol Lett. 2019 Oct 1;313:150-158.  [Content Brief]