BDNF Protein, Mouse (R129A, R130A, HEK293, His, Solution)

Brain Derived Neurotrophic Factor (BDNF) is a neurotrophin that belongs to NGF-beta family. BDNF can bind to its high affinity receptor TrkB and activates signal transduction cascades (IRS1/2, PI3K, Akt)^[1]. BDNF can also bind to the p75NTR, but the affinity for the p75NTR receptor is lower than for TrkB^[2]. BDNF is a neurotransmitter modulator, and is vital in maturation, survival and differentiation of neuronal populations during development. BDNF also participates in neuronal plasticity, which is essential for learning and memory. BDNF is widely expressed in the CNS^[1]. BDNF Protein, Mouse (R129A, R130A, HEK293, His) is a recombinant mouse BDNF (M1-R249, R129A, R130A) with C-terminal His tag, which is produced in HEK293.

BDNF, a neurotrophin that belongs to NGF-beta family. BDNF is widely expressed in the CNS, gut and other tissues. BDNF regulates neurodevelopmental processes, including maturation, survival and differentiation of neuronal populations, and synaptic plasticity^[1].
BDNF can bind to its high affinity receptor TrkB and activates signal transduction cascades (IRS1/2, PI3K, Akt), thereby inducing increased Ca²⁺ intake and phosphorylation of transcription factors. BDNF can also bind to the p75NTR, but the affinity for the p75NTR receptor is lower than for TrkB. The activation of p75NTR increases apoptotic and inflammatory signaling in neurons and glial cells by activation of c-Jun N-terminal kinases (JNK) and NF-κB expression, respectively^[2]. In BDNF knockout mouse, recombinant BDNF rescues deficits in basal synaptic transmission and hippocampal LTP^[3]. Mouse BDNF shares >97% aa sequence identity with human. Mouse BDNF shares >99% aa sequence identity with rat.
BDNF is a neurotransmitter modulator which is vital in maturation, survival and differentiation of neuronal populations during development. BDNF also participates in neuronal plasticity, which is essential for learning and memory^[1].

BDNF (mouse, 1 ng/mL, 3 min-2 h hippocampal slices from BDNF knockout mice) significantly improves deficits in basal synaptic transmission at the Schaffer collateral–CA1 synapse ^[3].

BDNF (mouse, 0.25 μg/side, intrahippocampal administration) mimics the beneficial effects (improve cognition) of NSC transplantation in AD mice^[4].

Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED₅₀ for this effect is 4.391 ng/mL, corresponding to a specific activity is 2.277×10⁵ units/mg.

Mouse

HEK293

C-His

P21237-1 (A19-R249, R129A, R130A)

12064  [NCBI]

Approximately 27.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Bathina S, et al. Brain-derived neurotrophic factor and its clinical implications. Arch Med Sci. 2015 Dec 10;11(6):1164-78.  [Content Brief]

  [2]. Lima Giacobbo B, et al. Brain-Derived Neurotrophic Factor in Brain Disorders: Focus on Neuroinflammation. Mol Neurobiol. 2019 May;56(5):3295-3312.  [Content Brief]

  [3]. Patterson SL, et al. Recombinant BDNF rescues deficits in basal synaptic transmission and hippocampal LTP in BDNF knockout mice. Neuron. 1996 Jun;16(6):1137-45.  [Content Brief]

  [4]. Blurton-Jones M, et al. Neural stem cells improve cognition via BDNF in a transgenic model of Alzheimer disease. Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13594-9.  [Content Brief]