BSP2 Protein, Mouse (HEK293, His)

BSP2 Protein, Mouse (HEK293, His) is a recombinant mouse bone sialoprotein 2 produced in HEK293 cells. Bone Sialoprotein 2, one of the major bone matrix proteins, with its previously known role in osseous tissue degradation, has been recently proven to play a role in the nucleation of hydroxyl apatite during osseous tissue mineralization^[1].

Bone Sialoprotein (BSP) is a member of the "Small Integrin-Binding Ligand N-linked Glycoproteins" (SIBLING) extracellular matrix protein family of mineralized tissues. BSP has been less studied than other SIBLING proteins such as Osteopontin (OPN), which is coexpressed with it in several skeletal cell types^[2].

Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast cancer cells. When 5×10⁴ cells/well are added to mouse BSP2 coated plates (3 µg/mL with 100 µL/well), approximately 60.05% will adhere after 30 minutes at 37 °C.

Mouse

HEK293

C-6*His

AAA21726.1 (F17-Q324)

15891  [NCBI]

FSMKNFHRRIKAEDSEENGVFKYRPRYFLYKHAYFYPPLKRFPVQGGSDSSEENGDGDSSEEEGEEEETSNEEENNEDSEGNEDQEAEAENATLSTLSGVTASYGAETTPQAQTFELAALQLPKKAGDAESRAPKVKESDEEEEEEEEEEENENEEAEVDENELAVNGTSTNSTEVDGGNGSSGGDNGEEAEAEEASVTEAGAEGTTGGRELTSVGTQTAVLLNGFQQTTPPPEAYGTTSPPIRKSSTVEYGGEYEQTGNEYNNEYEVYDNENGEPRGDTYRAYEDEYSYYKGHGYEGYEGQNYYYHQ

Approximately 60-90 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Im BJ, et al. Promotion of osteoblastic differentiation and osteogenic transcription factor expression on a microgroove titanium surface with immobilized fibronectin or bone sialoprotein II. Biomed Mater. 2016;11(3):035020. Published 2016 Jun 21.  [Content Brief]

  [2]. Bouleftour W, et al. The role of the SIBLING, Bone Sialoprotein in skeletal biology - Contribution of mouse experimental genetics. Matrix Biol. 2016;52-54:60-77.  [Content Brief]