Calumenin Protein, Human (HEK293, His)

Calumenin Protein, Human (HEK293, His), a recombinant human Calumenin produced in HEK293 cells, has a His tag at the N-terminus. Calumenin protein (CP) is located within the endoplasmic reticulum Ca²⁺ binding proteins. Calumenin as a suppressor of cell migration and tumor metastasis. Calumenin binds to and stabilizes fibulin-1, leading to inactivation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) signaling^[1][2].

Calumenin, belonging to the CREC protein family, is a multiple EF-hand Ca²⁺-binding protein, and has been found to have unique C-terminal SR retention signal HDEF. CP can combine with ryanodine receptor to active sarcoplasmic reticulum Ca²⁺ ATPase (SERCA2a) to regulate the release of intracellular calcium, intake, and storage of calcium in order to maintain homeostasis of calcium cycling. CP can also relieve ERS of myocardial cells and suppress ERS-mediated apoptosis. CP can relieve ERS-initiated apoptosis in viral myocarditis^[1].

Human

HEK293

C-6*His

O43852 (K20-F315)

813  [NCBI]

KPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDEFHHHHHH

43-55 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Yu Wang, et al. Calumenin relieves cardiac injury by inhibiting ERS-initiated apoptosis during viral myocarditis. Int J Clin Exp Pathol. 2017 Jul 1;10(7):7277-7284.  [Content Brief]

  [2]. Pengli Zheng, et al. Calumenin and fibulin-1 on tumor metastasis: Implications for pharmacology. Pharmacol Res. 2015 Sep;99:11-5.  [Content Brief]