VCAM-1/CD106 Protein, Human (HEK293, His)

CD106 Protein, Human (HEK293, His) is a cytokine-inducible cell surface protein capable of mediating adhesion.

CD106 (VCAM1) serves as a ligand for very late antigen-4 (VLA-4), which is present on leukocytes. CD106 (VCAM1) is a component of the neural stem cell niche that is critical for MSC-mediated immunosuppression and hematopoietic progenitor cell binding. CD106 promotes strong adhesion of leukocytes to the endothelium. Cell-to-cell adhesion mediated by CD106 is known to be critical for T-cell activation and leukocyte recruitment to inflammatory sites, and therefore it plays an important role in evoking effective immune responses. CD106 is highly expressed on chorionic villi (CV)-MSCs, moderately expressed on BM-MSCs, poorly expressed on umbilical cord MSCs, and not expressed on adipose MSCs^[1].

Human

HEK293

C-6*His

P19320 (F25-E698)

7412  [NCBI]

FKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTSTLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPEHHHHHH

85-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, 2 mM CaCl₂, 2 mM MgCl₂, 5% Threhalose, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Shihong Lu, et al. CD106 is a novel mediator of bone marrow mesenchymal stem cells via NF-κB in the bone marrow failure of acquired aplastic anemia. Stem Cell Res Ther. 2017 Aug 1;8(1):178.  [Content Brief]