1. Recombinant Proteins
  2. Immune Checkpoint Proteins Receptor Proteins Biotinylated Proteins
  3. Stimulatory Immune Checkpoint Molecules
  4. CD200R
  5. CD200R1
  6. CD200R1 Protein, Human (Biotinylated, HEK293, His-Avi)

CD200R1 Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P77601
Data Sheet Handling Instructions Technical Support

The CD200R1 protein is an inhibitory receptor that regulates key interactions by binding to the CD200/OX2 glycoprotein to limit inflammation by inhibiting TNF-α, interferon, and iNOS. Interestingly, CD200R1 displays comparable affinity and kinetics to the human herpesvirus 8 K14 viral CD200 homologue, reflecting its host CD200 interaction. CD200R1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD200R1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
20 μg USD 236 In-stock
50 μg USD 520 In-stock
100 μg USD 887 Get quote
> 100 μg   Get quote  

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  • Biological Activity

  • Technical Parameters

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Description

The CD200R1 protein is an inhibitory receptor that regulates key interactions by binding to the CD200/OX2 glycoprotein to limit inflammation by inhibiting TNF-α, interferon, and iNOS. Interestingly, CD200R1 displays comparable affinity and kinetics to the human herpesvirus 8 K14 viral CD200 homologue, reflecting its host CD200 interaction. CD200R1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD200R1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

Background

CD200R1, an inhibitory receptor, acts as a pivotal modulator by binding to the CD200/OX2 cell surface glycoprotein. Its regulatory role extends to limiting inflammation through the suppression of pro-inflammatory molecules, including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS), in response to specific stimuli. Intriguingly, CD200R1 exhibits comparable affinity and kinetics when binding to the Human herpesvirus 8 K14 viral CD200 homolog, mirroring its interaction with the host CD200. The interaction between CD200 and CD200R1 is facilitated through their respective N-terminal Ig-like domains, emphasizing the significance of this molecular interplay. Moreover, CD200R1 engages with the Human herpesvirus 8 vOX2 protein, further expanding its versatile interactions in cellular processes.

Biological Activity

Immobilized Biotinylated Human CD200 R1 at 1 μg/mL (100μL/Well) on the plate. Dose response curve for Human CD200 with the EC50 of 19.7 ng/mL determined by ELISA.

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

Q8TD46-4 (A27-L266)

Gene ID
Molecular Construction
N-term
CD200R1 (A27-L266)
Accession # Q8TD46-4
His-Avi
C-term
Synonyms
CD200R; CRTR2; MOX2R; OX2R; CD200 R1; CD200 receptor 1; HCRTR2; MOX2 receptor
Molecular Weight

52-70 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CD200R1 Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CD200R1 Protein, Human (Biotinylated, HEK293, His-Avi)
Cat. No.:
HY-P77601
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